TY - JOUR
T1 - Traumatic brain injury results in acute rarefication of the vascular network
AU - Obenaus, Andre
AU - Ng, Michelle
AU - Orantes, Amanda M.
AU - Kinney-Lang, Eli
AU - Rashid, Faisal
AU - Hamer, Mary
AU - DeFazio, Richard A.
AU - Tang, Jiping
AU - Zhang, John H.
AU - Pearce, William J.
N1 - Publisher Copyright:
© 2017 The Author(s).
PY - 2017/3/22
Y1 - 2017/3/22
N2 - The role of the cerebrovascular network and its acute response to TBI is poorly defined and emerging evidence suggests that cerebrovascular reactivity is altered. We explored how cortical vessels are physically altered following TBI using a newly developed technique, vessel painting. We tested our hypothesis that a focal moderate TBI results in global decrements to structural aspects of the vasculature. Rats (naïve, sham-operated, TBI) underwent a moderate controlled cortical impact. Animals underwent vessel painting perfusion to label the entire cortex at 1 day post TBI followed by whole brain axial and coronal images using a wide-field fluorescence microscope. Cortical vessel network characteristics were analyzed for classical angiographic features (junctions, lengths) wherein we observed significant global (both hemispheres) reductions in vessel junctions and vessel lengths of 33% and 22%, respectively. Biological complexity can be quantified using fractal geometric features where we observed that fractal measures were also reduced significantly by 33%, 16% and 13% for kurtosis, peak value frequency and skewness, respectively. Acutely after TBI there is a reduction in vascular network and vascular complexity that are exacerbated at the lesion site and provide structural evidence for the bilateral hemodynamic alterations that have been reported in patients after TBI.
AB - The role of the cerebrovascular network and its acute response to TBI is poorly defined and emerging evidence suggests that cerebrovascular reactivity is altered. We explored how cortical vessels are physically altered following TBI using a newly developed technique, vessel painting. We tested our hypothesis that a focal moderate TBI results in global decrements to structural aspects of the vasculature. Rats (naïve, sham-operated, TBI) underwent a moderate controlled cortical impact. Animals underwent vessel painting perfusion to label the entire cortex at 1 day post TBI followed by whole brain axial and coronal images using a wide-field fluorescence microscope. Cortical vessel network characteristics were analyzed for classical angiographic features (junctions, lengths) wherein we observed significant global (both hemispheres) reductions in vessel junctions and vessel lengths of 33% and 22%, respectively. Biological complexity can be quantified using fractal geometric features where we observed that fractal measures were also reduced significantly by 33%, 16% and 13% for kurtosis, peak value frequency and skewness, respectively. Acutely after TBI there is a reduction in vascular network and vascular complexity that are exacerbated at the lesion site and provide structural evidence for the bilateral hemodynamic alterations that have been reported in patients after TBI.
KW - Animals
KW - Blood Vessels/pathology
KW - Angiography
KW - Cerebral Cortex/pathology
KW - Cerebrovascular Circulation/physiology
KW - Brain Injuries, Traumatic/complications
KW - Rats
KW - Biometry
KW - Microscopy, Fluorescence
KW - Disease Models, Animal
UR - http://www.scopus.com/inward/record.url?scp=85021173868&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85021173868&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/1f6dff4d-afe8-3eaa-8732-9b1ce7391d4b/
U2 - 10.1038/s41598-017-00161-4
DO - 10.1038/s41598-017-00161-4
M3 - Article
C2 - 28331228
SN - 2045-2322
VL - 7
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 239
ER -