@inproceedings{5e54160b19a64c44853751dfe8198167,
title = "Time course of oxyhemoglobin induces apoptosis in mice brain cells in vivo",
abstract = "Several reports show that oxyHb can also directly induce injury of cells in vitro, but the time course of oxyHb's effect on brain cells in vivo is still unclear. Seventy-five Institution of Cancer Research (ICR) mice were randomly divided into an experimental group (n=40) and control group (n=35). Each group was then divided into 5 subgroups according to different post operational time (3, 6, 12, 24 and 48 h). Freshly made OxyHb was injected into the subarachnoid space of mice. The temporal and spatial distribution pattern of neurocytes necrosis and apoptosis in brain was examined using H \& E, TUNEL staining and transmission electron microscopy. Apoptosis was identified as the main pattern of cell death. Apoptotic cells were located mainly in the ipsilateral neocortex around the oxyHb injected region and the bilateral hippocampus. The numbers of apoptosis cells increased in 3 h and 6 h, and then decreased with time, to the lowest level at 48 h after oxyHb injection. Apoptotic bodies containing debris of nuclei and cytoplasm were also found. Our data show that oxyHb can directly induce brain injury that is mainly exhibited as apoptosis in vivo. {\textcopyright} 2008 Springer-Verlag.",
keywords = "Oxyhemoglobin, apoptosis, in vivo, subarachnoid haemorrhage",
author = "Wei Shi and Huang, \{L. Y.\} and Wang, \{R. Z.\} and Sun, \{J. J.\} and Wang, \{F. R.\} and Liu, \{C. X.\} and L. Zhou and Zhang, \{J. H.\}",
note = "Funding Information: This report is supported by Natural Science Foundation of China (No: 30270481).",
year = "2008",
month = mar,
day = "25",
doi = "10.1007/978-3-211-75718-5\_5",
language = "English",
isbn = "9783211757178",
series = "Acta Neurochirurgica, Supplementum",
publisher = "Springer Vienna",
number = "104",
pages = "23--26",
booktitle = "Cerebral Vasospasm",
}