TY - JOUR
T1 - The use of human embryonic kidney (HEK 293) cells to enhance characterization of the LMX1B pathway
AU - Stump, Robert Patrick
AU - Stump, Robert Patrick
AU - Feenstra, Jennifer M
AU - Castillo, Michael A
AU - Soriano, Salvador
AU - Oberg, Kerby C
N1 - LMX1B is a homeodomain transcription factor critical to kidney development and glomerular filtration. In humans, LMX1B haploinsufficiency causes a condition known as Nail Patella syndrome (NPS). Individuals with NPS typically have under developed nails, absent patellae, and impaired kidney function. In Lmx1b knockout mice, the absence of Lmx1b function disrupts glomerular filtration and abates urine production.
PY - 2012/4/1
Y1 - 2012/4/1
N2 - LMX1B is a homeodomain transcription factor critical to kidney development and glomerular filtration. In humans, LMX1B haploinsufficiency causes a condition known as Nail Patella syndrome (NPS). Individuals with NPS typically have under developed nails, absent patellae, and impaired kidney function. In Lmx1b knockout mice, the absence of Lmx1b function disrupts glomerular filtration and abates urine production. The molecules targeted by LMX1B that regulate glomerular development and function, however, are poorly characterized. Immortalized human embryonic kidney (HEK 293) cells provide a potential tool for high‐throughput identification of LMX1B targets and an in vitro system for dissecting the functional role of targets. Thus, we characterized the known LMX1B pathway in HEK 293 cells and evaluated sites of LMX1B binding. We demonstrate the expression of LMX1B and its renal targets, COLLAGEN 4 ALPHA 4 and PODICIN in HEK 293 cells by RT‐PCR. Protein expression of LMX1B was verified by western blot analysis. In addition, using Chromatin immunoprecipitation we determined that LMX1B binds to regulatory sequences associated with both target genes. Further characterization of the LMX1B pathway in HEK 293 cells is underway and will likely enhance the identification of relevant downstream targets and the molecular cascade that regulates glomerular development and function. Grant Funding Source : (NICHD) HD 39421
AB - LMX1B is a homeodomain transcription factor critical to kidney development and glomerular filtration. In humans, LMX1B haploinsufficiency causes a condition known as Nail Patella syndrome (NPS). Individuals with NPS typically have under developed nails, absent patellae, and impaired kidney function. In Lmx1b knockout mice, the absence of Lmx1b function disrupts glomerular filtration and abates urine production. The molecules targeted by LMX1B that regulate glomerular development and function, however, are poorly characterized. Immortalized human embryonic kidney (HEK 293) cells provide a potential tool for high‐throughput identification of LMX1B targets and an in vitro system for dissecting the functional role of targets. Thus, we characterized the known LMX1B pathway in HEK 293 cells and evaluated sites of LMX1B binding. We demonstrate the expression of LMX1B and its renal targets, COLLAGEN 4 ALPHA 4 and PODICIN in HEK 293 cells by RT‐PCR. Protein expression of LMX1B was verified by western blot analysis. In addition, using Chromatin immunoprecipitation we determined that LMX1B binds to regulatory sequences associated with both target genes. Further characterization of the LMX1B pathway in HEK 293 cells is underway and will likely enhance the identification of relevant downstream targets and the molecular cascade that regulates glomerular development and function. Grant Funding Source : (NICHD) HD 39421
UR - https://www.fasebj.org/doi/abs/10.1096/fasebj.26.1_supplement.909.5
U2 - 10.1096/fasebj.26.1_supplement.909.5
DO - 10.1096/fasebj.26.1_supplement.909.5
M3 - Article
VL - 26
JO - The FASEB Journal
JF - The FASEB Journal
IS - S1
ER -