The Long-Term Effect of Saline and Phosphate Buffer Solution on MTA: An SEM and EPMA Investigation

Masoud Parirokh; Saeed Asgary; Mohammad Jafar Eghbal; Jamileh Ghoddusi; Frank Brink; Sara Askarifar; Mahmoud Torabinejad; Maryam Raoof

doi:10.22037/iej.v2i3.309

The Long-Term Effect of Saline and Phosphate Buffer Solution on MTA: An SEM and EPMA Investigation

Masoud Parirokh
, Saeed Asgary
, Mohammad Jafar Eghbal
, Jamileh Ghoddusi
, Frank Brink
, Sara Askarifar
, Mahmoud Torabinejad
, Maryam Raoof

Endodontics

Research output: Contribution to journal › Article › peer-review

Abstract

<div class="line" id="line-5"> <b style="font-family: Tahoma, sans-serif; font-size: 10pt;"> Introduction </b> <span style="font-family: Tahoma, sans-serif; font-size: 10pt;"> : To examine the long-term effects of normal saline and a synthetic tissue fluid (phosphate buffered saline, PBS) on mineral trioxide aggregate (MTA). </span></div><div class="line" id="line-200"> <br/></div><div class="line" id="line-39"> <span style="font-family: Tahoma, sans-serif; font-size: 10pt;"> <b> Materials and Methods </b> : Root-ends of twelve extracted human teeth were resected; root-end cavities were prepared and filled with MTA. Samples were randomly divided into two groups of six each. Teeth in <span class="GINGER_SOFTWARE_mark" ginger_software_uiphraseguid="bc6dd27d-5ccc-47c7-a215-0defc379ec80" id="3b67553f-18fd-4314-a667-13a4f803392a"> group </span> I <span class="GINGER_SOFTWARE_mark" ginger_software_uiphraseguid="bc6dd27d-5ccc-47c7-a215-0defc379ec80" id="78114211-ac74-4bb0-a830-b2fa39ac73ff"> were placed </span> in normal saline, whilst teeth in group 11 were placed in PBS. After five months, elemental analysis of the surface of the root-end filling was performed using electron probe <span class="GINGER_SOFTWARE_mark" ginger_software_uiphraseguid="baf88cf2-9c3d-4f22-afed-20947798a979" id="b6475cda-b127-4d50-93df-278e6a75ca3a"> microanalysis </span> (EPMA). </span></div><div class="line" id="line-163"> <br/></div><div class="line" id="line-44"> <span style="font-family: Tahoma, sans-serif; font-size: 10pt;"> <b> Results </b> : Results showed that a <span class="GINGER_SOFTWARE_mark" ginger_software_uiphraseguid="7f555322-d04c-4634-8339-fb265276f64d" id="7b87735b-256a-4ba0-afce-4dd0cf6e2c1b"> teeth </span> kept in PBS formed crystal deposits. In contrast, no such crystal formation was observed in teeth kept in normal saline solution. The results of elemental analysis showed that the composition of the crystals observed for teeth kept in PBS was consistent with that of a mineral <span class="GINGER_SOFTWARE_mark" ginger_software_uiphraseguid="2382fda5-cc9f-432a-ba41-0cb44a226fb5" id="7d1616bc-3f4f-4b83-9a9f-608b12f49f0d"> hydroxyapatite </span> <span class="GINGER_SOFTWARE_mark" ginger_software_uiphraseguid="2382fda5-cc9f-432a-ba41-0cb44a226fb5" id="c2fc2304-83a2-4739-84b5-dfc9905cd0b7"> , </span> Ca10 <span class="GINGER_SOFTWARE_mark" ginger_software_uiphraseguid="2382fda5-cc9f-432a-ba41-0cb44a226fb5" id="42cfd167-55c1-4b47-a77a-af5016cff248"> ( </span> PO4MOH <span class="GINGER_SOFTWARE_mark" ginger_software_uiphraseguid="2382fda5-cc9f-432a-ba41-0cb44a226fb5" id="56bc1bfe-e697-4921-bd89-79f7298a7e5f"> ) </span> 2. </span></div><div class="line" id="line-156"> <br/></div><div class="line" id="line-49"> <span style="font-family: Tahoma, sans-serif; font-size: 10pt;"> <b> Conclusion </b> : Based on these in vitro results, we suggest that the hydration of MTA surface and the release of calcium from MTA in contact with phosphorous of PBS produced <span class="GINGER_SOFTWARE_mark" ginger_software_uiphraseguid="d10d65cd-98d4-4f96-b76d-9c3977a21805" id="20f0f008-7f8e-4eb1-955d-3e196a62f09f"> hydroxyapatite </span> crystals over MTA and it may be a mechanism which is responsible for cementum formation during in vivo studies. </span></div><div class="line" id="line-54">  </div>

Original language	American English
Journal	IRANIAN ENDODONTIC JOURNAL (IEJ)
Volume	2
Issue number	3
DOIs	https://doi.org/10.22037/iej.v2i3.309
State	Published - Oct 2007

Keywords

EPMA
MTA
PBS
RETRO FILLING MATERIAL
SEM
STORAGE MEDIA

Disciplines

Dentistry
Materials Science and Engineering
Biological Engineering
Mineral Physics

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@article{8e87e19719374b289f7b3d2feb985331,

title = "The Long-Term Effect of Saline and Phosphate Buffer Solution on MTA: An SEM and EPMA Investigation",

abstract = " Introduction : To examine the long-term effects of normal saline and a synthetic tissue fluid (phosphate buffered saline, PBS) on mineral trioxide aggregate (MTA). Materials and Methods : Root-ends of twelve extracted human teeth were resected; root-end cavities were prepared and filled with MTA. Samples were randomly divided into two groups of six each. Teeth in group I were placed in normal saline, whilst teeth in group 11 were placed in PBS. After five months, elemental analysis of the surface of the root-end filling was performed using electron probe microanalysis (EPMA). Results : Results showed that a teeth kept in PBS formed crystal deposits. In contrast, no such crystal formation was observed in teeth kept in normal saline solution. The results of elemental analysis showed that the composition of the crystals observed for teeth kept in PBS was consistent with that of a mineral hydroxyapatite , Ca10 ( PO4MOH ) 2. Conclusion : Based on these in vitro results, we suggest that the hydration of MTA surface and the release of calcium from MTA in contact with phosphorous of PBS produced hydroxyapatite crystals over MTA and it may be a mechanism which is responsible for cementum formation during in vivo studies. \ ",

keywords = "EPMA, MTA, PBS, RETRO FILLING MATERIAL, SEM, STORAGE MEDIA",

author = "Masoud Parirokh and Saeed Asgary and Eghbal, \{Mohammad Jafar\} and Jamileh Ghoddusi and Frank Brink and Sara Askarifar and Mahmoud Torabinejad and Maryam Raoof",

year = "2007",

month = oct,

doi = "10.22037/iej.v2i3.309",

language = "American English",

volume = "2",

journal = "IRANIAN ENDODONTIC JOURNAL (IEJ)",

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T1 - The Long-Term Effect of Saline and Phosphate Buffer Solution on MTA: An SEM and EPMA Investigation

AU - Parirokh, Masoud

AU - Asgary, Saeed

AU - Eghbal, Mohammad Jafar

AU - Ghoddusi, Jamileh

AU - Brink, Frank

AU - Askarifar, Sara

AU - Torabinejad, Mahmoud

AU - Raoof, Maryam

PY - 2007/10

Y1 - 2007/10

N2 - Introduction : To examine the long-term effects of normal saline and a synthetic tissue fluid (phosphate buffered saline, PBS) on mineral trioxide aggregate (MTA). Materials and Methods : Root-ends of twelve extracted human teeth were resected; root-end cavities were prepared and filled with MTA. Samples were randomly divided into two groups of six each. Teeth in group I were placed in normal saline, whilst teeth in group 11 were placed in PBS. After five months, elemental analysis of the surface of the root-end filling was performed using electron probe microanalysis (EPMA). Results : Results showed that a teeth kept in PBS formed crystal deposits. In contrast, no such crystal formation was observed in teeth kept in normal saline solution. The results of elemental analysis showed that the composition of the crystals observed for teeth kept in PBS was consistent with that of a mineral hydroxyapatite , Ca10 ( PO4MOH ) 2. Conclusion : Based on these in vitro results, we suggest that the hydration of MTA surface and the release of calcium from MTA in contact with phosphorous of PBS produced hydroxyapatite crystals over MTA and it may be a mechanism which is responsible for cementum formation during in vivo studies.  

AB - Introduction : To examine the long-term effects of normal saline and a synthetic tissue fluid (phosphate buffered saline, PBS) on mineral trioxide aggregate (MTA). Materials and Methods : Root-ends of twelve extracted human teeth were resected; root-end cavities were prepared and filled with MTA. Samples were randomly divided into two groups of six each. Teeth in group I were placed in normal saline, whilst teeth in group 11 were placed in PBS. After five months, elemental analysis of the surface of the root-end filling was performed using electron probe microanalysis (EPMA). Results : Results showed that a teeth kept in PBS formed crystal deposits. In contrast, no such crystal formation was observed in teeth kept in normal saline solution. The results of elemental analysis showed that the composition of the crystals observed for teeth kept in PBS was consistent with that of a mineral hydroxyapatite , Ca10 ( PO4MOH ) 2. Conclusion : Based on these in vitro results, we suggest that the hydration of MTA surface and the release of calcium from MTA in contact with phosphorous of PBS produced hydroxyapatite crystals over MTA and it may be a mechanism which is responsible for cementum formation during in vivo studies.  

KW - EPMA

KW - MTA

KW - PBS

KW - RETRO FILLING MATERIAL

KW - SEM

KW - STORAGE MEDIA

UR - http://www.sid.ir/en/VEWSSID/J_pdf/109620070302.pdf

U2 - 10.22037/iej.v2i3.309

DO - 10.22037/iej.v2i3.309

M3 - Article

VL - 2

JO - IRANIAN ENDODONTIC JOURNAL (IEJ)

JF - IRANIAN ENDODONTIC JOURNAL (IEJ)

IS - 3

ER -

The Long-Term Effect of Saline and Phosphate Buffer Solution on MTA: An SEM and EPMA Investigation

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