TY - JOUR
T1 - Specific binding of estrogen to nuclei from japanese quail kidney cells
AU - Turner, R. T.
AU - Howard, G. A.
AU - Baylink, D. J.
N1 - Funding Information:
We thank M i s s Lucy Dillman for excellent technical assistance. This work was supported by NIH grant AG 00299 and by the Research Service of the Veterans Administration.
PY - 1978
Y1 - 1978
N2 - The binding properties of estrogen to Japanese quail kidney cell nuclei were evaluated by 3H-17βestradiol exchange. Kidney cell nuclei from estrogenized male, as well as laying female quail, had a single class of saturable (0.1-0.2 pmol/mg DNA), high affinity (Kd = 1-2 × 10-10M) nuclear binding sites for 17βestradiol. High affinity estrogen binding sites were not found in kidney cell nuclei from untreated males. Various non-radioactive estrogens (17βestradiol, estrone, estriol and diethylstilbestrol) competed effectively with 3H-17βestradiol for the high affinity binding sites, whereas nonestrogens (testosterone and progesterone) did not compete. It is concluded that the 3H-estradiol binding characteristics of kidney cell nuclei provides strong evidence for a nuclear estrogen receptor in the avian kidney.
AB - The binding properties of estrogen to Japanese quail kidney cell nuclei were evaluated by 3H-17βestradiol exchange. Kidney cell nuclei from estrogenized male, as well as laying female quail, had a single class of saturable (0.1-0.2 pmol/mg DNA), high affinity (Kd = 1-2 × 10-10M) nuclear binding sites for 17βestradiol. High affinity estrogen binding sites were not found in kidney cell nuclei from untreated males. Various non-radioactive estrogens (17βestradiol, estrone, estriol and diethylstilbestrol) competed effectively with 3H-17βestradiol for the high affinity binding sites, whereas nonestrogens (testosterone and progesterone) did not compete. It is concluded that the 3H-estradiol binding characteristics of kidney cell nuclei provides strong evidence for a nuclear estrogen receptor in the avian kidney.
UR - https://www.scopus.com/pages/publications/0018240690
UR - https://www.scopus.com/pages/publications/0018240690#tab=citedBy
U2 - 10.1080/07435807809061097
DO - 10.1080/07435807809061097
M3 - Article
C2 - 752636
SN - 0743-5800
VL - 5
SP - 335
EP - 342
JO - Endocrine Research
JF - Endocrine Research
IS - 4
ER -