Single-Cell Digital Lysates Generated by Phase-Switch Microfluidic Device Reveal Transcriptome Perturbation of Cell Cycle

Yan Chen, Joshua Millstein, Yao Liu, Gina Y. Chen, Xuelian Chen, Andres Stucky, Cunye Qu, Jian Bing Fan, Xiao Chang, Ava Soleimany, Kai Wang, Jiangjian Zhong, Jie Liu, Frank D. Gilliland, Zhongjun Li, Xi Zhang, Jiang F. Zhong

Research output: Contribution to journalArticlepeer-review

Original languageEnglish
Pages (from-to)4687-4694
Number of pages8
JournalACS Nano
Volume12
Issue number5
DOIs
StatePublished - May 22 2018
Externally publishedYes

ASJC Scopus Subject Areas

  • General Materials Science
  • General Engineering
  • General Physics and Astronomy

Keywords

  • digital lysate
  • human embryonic stem cell
  • microfluidic
  • single cell
  • transcriptome
  • Microfluidic Analytical Techniques/methods
  • Single-Cell Analysis/methods
  • Cell Cycle/physiology
  • Human Embryonic Stem Cells/physiology
  • Humans
  • Lab-On-A-Chip Devices
  • Transcriptome/genetics
  • RNA, Small Interfering/analysis
  • High-Throughput Nucleotide Sequencing/methods

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