TY - JOUR
T1 - Sheep platelets as a model for human platelets
T2 - Evidence for specific paf (platelet activating factor) receptors
AU - Slattery, Charles W.
AU - Beaumont, Duane O.
N1 - Funding Information:
This research was supported in part by a grant from the American Heart Association, California Affiliate.
PY - 1989/9/1
Y1 - 1989/9/1
N2 - In a number of animals, the platelet response to Platelet Activating Factor (PAF) has been shown to differ considerably from that in humans. However, aggregation, release and particularly shape change were quite similar for human and sheep platelets. In this study, aggregation and shape change analysis were used to measure the response of sheep platelets to various synthetic analogs of PAF. Response is greatly reduced with no alkyl group in position 2 of PAF and decreases progressively as the number of carbons of the alkyl gets larger than three. A reduction of activity is also seen as the ether linkage at position 1 of PAF is replaced by an ester linkage. These changes are indicative of a specific membrane receptor for PAF in sheep platelets and confirm the usefulness of sheep platelets as a model for PAF-platelet interaction in humans.
AB - In a number of animals, the platelet response to Platelet Activating Factor (PAF) has been shown to differ considerably from that in humans. However, aggregation, release and particularly shape change were quite similar for human and sheep platelets. In this study, aggregation and shape change analysis were used to measure the response of sheep platelets to various synthetic analogs of PAF. Response is greatly reduced with no alkyl group in position 2 of PAF and decreases progressively as the number of carbons of the alkyl gets larger than three. A reduction of activity is also seen as the ether linkage at position 1 of PAF is replaced by an ester linkage. These changes are indicative of a specific membrane receptor for PAF in sheep platelets and confirm the usefulness of sheep platelets as a model for PAF-platelet interaction in humans.
KW - Platelet Activating Factor
KW - Platelet Receptors
KW - Sheep Platelets
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U2 - 10.1016/0049-3848(89)90389-7
DO - 10.1016/0049-3848(89)90389-7
M3 - Article
C2 - 2554526
SN - 0049-3848
VL - 55
SP - 569
EP - 576
JO - Thrombosis Research
JF - Thrombosis Research
IS - 5
ER -