TY - JOUR
T1 - Restoration of Cdk5, Trkb and soluble N-ethylmaleimide-sensitive factor attachment protein receptor proteins after chronic methylphenidate treatment in spontaneous hypertensive rats, a model for attention-deficit hyperactivity disorder
AU - Kim, Yeni
AU - Jeon, Songhee
AU - Jeong, Ha Jin
AU - Lee, Seong Mi
AU - Peña, Ike Dela
AU - Kim, Hee Jin
AU - Han, Doug Hyun
AU - Kim, Bung Nyun
AU - Cheong, Jae Hoon
N1 - Publisher Copyright:
© 2019 Korean Neuropsychiatric Association.
PY - 2019/7
Y1 - 2019/7
N2 - Objective Synaptic vesicle mobilization and neurite outgrowth regulation molecules were examined in modulation of effects of methylphenidate (MPH) in Spontaneous Hypertensive Rats (SHRs), a model for attention-deficit hyperactivity disorder (ADHD). Methods We compared the changes in the protein expression level of Cyclin dependent kinase 5 (Cdk5) and molecular substrates of Cdk5; tropomyosin receptor kinase B (TrkB), syntaxin 1A (STX1A) and synaptosomal-associated protein 25 (SNAP25). Comparisons were made in prefrontal cortex of vehicle (distilled water i.p. for 7 days)-treated SHRs, vehicle-treated Wistar Kyoto Rats (WKYs) and MPH (2 mg/kg i.p. for 7 days) treated SHRs. Results The Cdk5 level of vehicle-treated SHRs was significantly decreased compared to the Cdk5 level of vehicle-treated WKY rats, but was restored to the expression level of vehicle-treated WKYs in MPH-treated SHR. The ratio of p25/p35 was significantly decreased in MPH-treated SHR compared to vehicle-treated SHR. Moreover, TrkB, STX1A and SNAP25 of vehicle-treated SHRs were significantly decreased compared to vehicle-treated WKY rats, but were restored to the expression level of vehicle-treated WKYs in MPH-treated SHR. Conclusion The results show that Cdk5, TrkB, STX1A, and SNAP25 were involved in the modulation of MPH effects in prefrontal cortex of SHRs and play important role in treatment of ADHD.
AB - Objective Synaptic vesicle mobilization and neurite outgrowth regulation molecules were examined in modulation of effects of methylphenidate (MPH) in Spontaneous Hypertensive Rats (SHRs), a model for attention-deficit hyperactivity disorder (ADHD). Methods We compared the changes in the protein expression level of Cyclin dependent kinase 5 (Cdk5) and molecular substrates of Cdk5; tropomyosin receptor kinase B (TrkB), syntaxin 1A (STX1A) and synaptosomal-associated protein 25 (SNAP25). Comparisons were made in prefrontal cortex of vehicle (distilled water i.p. for 7 days)-treated SHRs, vehicle-treated Wistar Kyoto Rats (WKYs) and MPH (2 mg/kg i.p. for 7 days) treated SHRs. Results The Cdk5 level of vehicle-treated SHRs was significantly decreased compared to the Cdk5 level of vehicle-treated WKY rats, but was restored to the expression level of vehicle-treated WKYs in MPH-treated SHR. The ratio of p25/p35 was significantly decreased in MPH-treated SHR compared to vehicle-treated SHR. Moreover, TrkB, STX1A and SNAP25 of vehicle-treated SHRs were significantly decreased compared to vehicle-treated WKY rats, but were restored to the expression level of vehicle-treated WKYs in MPH-treated SHR. Conclusion The results show that Cdk5, TrkB, STX1A, and SNAP25 were involved in the modulation of MPH effects in prefrontal cortex of SHRs and play important role in treatment of ADHD.
KW - Cyclin dependent kinase 5
KW - Methylphenidate
KW - Synaptosomal-associated protein 25
KW - Syntaxin 1A
KW - Tropomyosin receptor kinase B
UR - https://www.scopus.com/pages/publications/85071696242
UR - https://www.scopus.com/pages/publications/85071696242#tab=citedBy
UR - https://www.mendeley.com/catalogue/a03d8ea1-d922-38e4-ab74-79404bc994be/
U2 - 10.30773/pi.2019.04.22
DO - 10.30773/pi.2019.04.22
M3 - Article
SN - 1738-3684
VL - 16
SP - 558
EP - 564
JO - Psychiatry Investigation
JF - Psychiatry Investigation
IS - 7
ER -