Remote ischemic postconditioning (RIPC) after GMH in rodents

Tim Lekic, Damon Klebe, Jerry Flores, Regina Peters, William B. Rolland, Jiping Tang, John H. Zhang

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)peer-review

Abstract

Germinal matrix hemorrhage (GMH) is the most common and devastating neurological injury of premature infants, and current treatment approaches are ineffective. Remote ischemic postconditioning (RIPC) is a method by which brief limb ischemic stimuli protect the injured brain. We hypothesized that RIPC can improve outcomes following GMH in rats. Neonatal rats (P7) were subjected to either stereotactic ganglionic eminence collagenase infusion or sham surgery. Groups were as follows: sham (n = 0), GMH non-RIPC (n = 10), GMH + 1 week RIPC (n = 10), GMH + 2 weeks RIPC (n = 10). Neurobehavior analysis at the fourth week consisted of Morris water maze (MWM) and rotarod (RR). This was followed by euthanasia for histopathology on day 28. Both 1- and 2-week RIPC showed significant improvement in FF and RR motor testing compared with untreated animals (i.e., GMH without RIPC). RIPC treatment also improved cognition (MWM) and attenuated neuropathological ventricular enlargement (hydrocephalus) in juvenile animals following GMH. RIPC is a safe and noninvasive approach that improved sensorimotor and neuropathological outcomes following GMH in rats. Further studies are needed to evaluate for mechanisms of neuroprotection.

Original languageEnglish
Title of host publicationBrain Edema XVI
PublisherSpringer Cham
Pages63-67
Number of pages5
ISBN (Electronic)978-3-319-18497-5
ISBN (Print)978-3-319-18496-8, 978-3-319-36532-9
DOIs
StatePublished - Jan 1 2016

Publication series

NameActa Neurochirurgica, Supplementum
Volume121
ISSN (Print)0065-1419
ISSN (Electronic)2197-8395

ASJC Scopus Subject Areas

  • Surgery
  • Clinical Neurology

Keywords

  • Animals, Newborn
  • Cerebral Hemorrhage/chemically induced
  • Rats
  • Random Allocation
  • Rats, Sprague-Dawley
  • Brain/physiopathology
  • Behavior, Animal
  • Rotarod Performance Test
  • Animals
  • Maze Learning
  • Microbial Collagenase/toxicity
  • Ischemic Postconditioning/methods
  • Extremities
  • Disease Models, Animal

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