Quantitation of growth factors in ossein-mineral-compound

Jan J. Stepan; Subburaman Mohan; John C. Jennings; Jon E. Wergedal; Arch K. Taylor; David J. Baylink

doi:10.1016/0024-3205(91)90083-N

Quantitation of growth factors in ossein-mineral-compound

Jan J. Stepan
, Subburaman Mohan
, John C. Jennings
, Jon E. Wergedal
, Arch K. Taylor
, David J. Baylink

Research output: Contribution to journal › Article › peer-review

Abstract

Study was undertaken to identify polypeptide factors in the commercially available ossein-mineral-compound and to see if they are present in a biologically relevant quantity. Using the guanidine-EDTA extraction, 35.7 ± 0.1 mg proteins were obtained from 1 g of the ossen-mineral-compound. At concentration 1 μg/ml, guanidine-EDTA-extractable proteins stimulated the incorporation of thymidine into DNA by human bone cells to 581 ± 122 % (p<0.001) of that by bovine serum albumin-treated control cells, decreasing thereafter. Similarly, it stimulated the activity of alkaline phosphatase in the human bone cells. Growth factors IGF-I, IGF-II, and TGF-β were identified in the ossein-mineral-compound. This leads to speculation regarding possible role of growth factors in explaining the beneficial effects of the compound in retarding bone loss in patients with osteoporosis.

Original language	English
Pages (from-to)	PL79-PL84
Journal	Life Sciences
Volume	49
Issue number	13
DOIs	https://doi.org/10.1016/0024-3205(91)90083-N
State	Published - 1991

ASJC Scopus Subject Areas

General Biochemistry,Genetics and Molecular Biology
General Pharmacology, Toxicology and Pharmaceutics

Access to Document

10.1016/0024-3205(91)90083-NLicense: Unspecified

OpenUrl availability

Full text

Cite this

@article{a4f61be984c249c49676e29aad7bd998,

title = "Quantitation of growth factors in ossein-mineral-compound",

abstract = "Study was undertaken to identify polypeptide factors in the commercially available ossein-mineral-compound and to see if they are present in a biologically relevant quantity. Using the guanidine-EDTA extraction, 35.7 ± 0.1 mg proteins were obtained from 1 g of the ossen-mineral-compound. At concentration 1 μg/ml, guanidine-EDTA-extractable proteins stimulated the incorporation of thymidine into DNA by human bone cells to 581 ± 122 \% (p<0.001) of that by bovine serum albumin-treated control cells, decreasing thereafter. Similarly, it stimulated the activity of alkaline phosphatase in the human bone cells. Growth factors IGF-I, IGF-II, and TGF-β were identified in the ossein-mineral-compound. This leads to speculation regarding possible role of growth factors in explaining the beneficial effects of the compound in retarding bone loss in patients with osteoporosis.",

author = "Stepan, \{Jan J.\} and Subburaman Mohan and Jennings, \{John C.\} and Wergedal, \{Jon E.\} and Taylor, \{Arch K.\} and Baylink, \{David J.\}",

note = "Study was undertaken to identify polypeptide factors in the commercially available ossein-mineral-compound and to see if they are present in a biologi...",

year = "1991",

doi = "10.1016/0024-3205(91)90083-N",

language = "English",

volume = "49",

pages = "PL79--PL84",

journal = "Life Sciences",

issn = "0024-3205",

number = "13",

}

TY - JOUR

T1 - Quantitation of growth factors in ossein-mineral-compound

AU - Stepan, Jan J.

AU - Mohan, Subburaman

AU - Jennings, John C.

AU - Wergedal, Jon E.

AU - Taylor, Arch K.

AU - Baylink, David J.

N1 - Study was undertaken to identify polypeptide factors in the commercially available ossein-mineral-compound and to see if they are present in a biologi...

PY - 1991

Y1 - 1991

N2 - Study was undertaken to identify polypeptide factors in the commercially available ossein-mineral-compound and to see if they are present in a biologically relevant quantity. Using the guanidine-EDTA extraction, 35.7 ± 0.1 mg proteins were obtained from 1 g of the ossen-mineral-compound. At concentration 1 μg/ml, guanidine-EDTA-extractable proteins stimulated the incorporation of thymidine into DNA by human bone cells to 581 ± 122 % (p<0.001) of that by bovine serum albumin-treated control cells, decreasing thereafter. Similarly, it stimulated the activity of alkaline phosphatase in the human bone cells. Growth factors IGF-I, IGF-II, and TGF-β were identified in the ossein-mineral-compound. This leads to speculation regarding possible role of growth factors in explaining the beneficial effects of the compound in retarding bone loss in patients with osteoporosis.

AB - Study was undertaken to identify polypeptide factors in the commercially available ossein-mineral-compound and to see if they are present in a biologically relevant quantity. Using the guanidine-EDTA extraction, 35.7 ± 0.1 mg proteins were obtained from 1 g of the ossen-mineral-compound. At concentration 1 μg/ml, guanidine-EDTA-extractable proteins stimulated the incorporation of thymidine into DNA by human bone cells to 581 ± 122 % (p<0.001) of that by bovine serum albumin-treated control cells, decreasing thereafter. Similarly, it stimulated the activity of alkaline phosphatase in the human bone cells. Growth factors IGF-I, IGF-II, and TGF-β were identified in the ossein-mineral-compound. This leads to speculation regarding possible role of growth factors in explaining the beneficial effects of the compound in retarding bone loss in patients with osteoporosis.

UR - https://www.scopus.com/pages/publications/0025823518

UR - https://www.scopus.com/pages/publications/0025823518#tab=citedBy

U2 - 10.1016/0024-3205(91)90083-N

DO - 10.1016/0024-3205(91)90083-N

M3 - Article

C2 - 1653384

SN - 0024-3205

VL - 49

SP - PL79-PL84

JO - Life Sciences

JF - Life Sciences

IS - 13

ER -

Quantitation of growth factors in ossein-mineral-compound

Abstract

ASJC Scopus Subject Areas

Access to Document

OpenUrl availability

Other files and links

Cite this