TY - JOUR
T1 - Purification and N-terminal sequence of two tartrate-resistant acid phosphatases type-5 from the hairy cell leukemia spleen
AU - Štěpán, J. J.
AU - Lau, K. H.W.
AU - Mohan, S.
AU - Kraenzlin, M.
AU - Baylink, D. J.
N1 - Tartrate-resistant acid phosphatases types 5a and 5b were purified from human hairy cell leukemia spleen by sequnetial chromatography on Phenyl-Sephar...
PY - 1989/12/30
Y1 - 1989/12/30
N2 - Tartrate-resistant acid phosphatases types 5a and 5b were purified from human hairy cell leukemia spleen by sequnetial chromatography on Phenyl-Sepharose, CM-Sepharose, concanavalin A-Sepharose, FPLC Superose-12 and FPLC Mono-S. The purification over the original tissue extract was 1150- and 3300-fold, with a yield of 2.1% and 2.5%, respectively. Gel filtration indicated an Mr of about 30000 for both forms. There was a N-terminal sequence identity between the two enzymes. However, they appeared to be different as assessed by cation exchange chromatography and amino acid composition.
AB - Tartrate-resistant acid phosphatases types 5a and 5b were purified from human hairy cell leukemia spleen by sequnetial chromatography on Phenyl-Sepharose, CM-Sepharose, concanavalin A-Sepharose, FPLC Superose-12 and FPLC Mono-S. The purification over the original tissue extract was 1150- and 3300-fold, with a yield of 2.1% and 2.5%, respectively. Gel filtration indicated an Mr of about 30000 for both forms. There was a N-terminal sequence identity between the two enzymes. However, they appeared to be different as assessed by cation exchange chromatography and amino acid composition.
UR - https://www.scopus.com/pages/publications/0024832401
UR - https://www.scopus.com/pages/publications/0024832401#tab=citedBy
U2 - 10.1016/0006-291X(89)92705-8
DO - 10.1016/0006-291X(89)92705-8
M3 - Article
C2 - 2610679
SN - 0006-291X
VL - 165
SP - 1027
EP - 1034
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -