Pulse-modulated second harmonic imaging microscope quantitatively demonstrates marked increase of collagen in tumor after chemotherapy

Anju M. Raja; Shuoyu Xu; Wanxin Sun; Jianbiao Zhou; Dean C.S. Tai; Chien Shing Chen; Jagath C. Rajapakse; Peter T.C. So; Hanry Yu

doi:10.1117/1.3497565

Pulse-modulated second harmonic imaging microscope quantitatively demonstrates marked increase of collagen in tumor after chemotherapy

Anju M. Raja
, Shuoyu Xu
, Wanxin Sun
, Jianbiao Zhou
, Dean C.S. Tai
, Chien Shing Chen
, Jagath C. Rajapakse
, Peter T.C. So
, Hanry Yu

Research output: Contribution to journal › Article › peer-review

Abstract

Pulse-modulated second harmonic imaging microscopes (PM-SHIMs) exhibit improved signal-to-noise ratio (SNR) over conventional SHIMs on sensitive imaging and quantification of weak collagen signals inside tissues. We quantify the spatial distribution of sparse collagen inside a xenograft model of human acute myeloid leukemia (AML) tumor specimens treated with a new drug against receptor tyrosine kinase (ABT-869), and observe a significant increase in collagen area percentage, collagen fiber length, fiber width, and fiber number after chemotherapy. This finding reveals new insights into tumor responses to chemotherapy and suggests caution in developing new drugs and therapeutic regimens against cancers.

Original language	English
Article number	056016
Journal	Journal of Biomedical Optics
Volume	15
Issue number	5
DOIs	https://doi.org/10.1117/1.3497565
State	Published - Sep 2010

ASJC Scopus Subject Areas

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Biomedical Engineering

Keywords

collagen modulation postchemotherapy
drug resistance
pulse modulation
second harmonic generation imaging

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title = "Pulse-modulated second harmonic imaging microscope quantitatively demonstrates marked increase of collagen in tumor after chemotherapy",

abstract = "Pulse-modulated second harmonic imaging microscopes (PM-SHIMs) exhibit improved signal-to-noise ratio (SNR) over conventional SHIMs on sensitive imaging and quantification of weak collagen signals inside tissues. We quantify the spatial distribution of sparse collagen inside a xenograft model of human acute myeloid leukemia (AML) tumor specimens treated with a new drug against receptor tyrosine kinase (ABT-869), and observe a significant increase in collagen area percentage, collagen fiber length, fiber width, and fiber number after chemotherapy. This finding reveals new insights into tumor responses to chemotherapy and suggests caution in developing new drugs and therapeutic regimens against cancers.",

keywords = "collagen modulation postchemotherapy, drug resistance, pulse modulation, second harmonic generation imaging",

author = "Raja, \{Anju M.\} and Shuoyu Xu and Wanxin Sun and Jianbiao Zhou and Tai, \{Dean C.S.\} and Chen, \{Chien Shing\} and Rajapakse, \{Jagath C.\} and So, \{Peter T.C.\} and Hanry Yu",

note = "Funding Information: This work is supported in part by the Institute of Bioengineering and Nanotechnology, BMRC, A*STAR of Singapore, and grants from BMRC (R185-001-045-305), Ministry of Education (R-185-000-135-112), Janssen-Cilag (R-185-000-182-592), Singapore-MIT Alliance Computational and Systems Biology Flagship Project funding (C-382-603-004-001), SMART BioSyM and Mechanobiology Institute, Singapore to Yu. Raja and Zhou are NUS Research Scholars, and Xu is a SMA Research Scholar.",

year = "2010",

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doi = "10.1117/1.3497565",

language = "English",

volume = "15",

journal = "Journal of Biomedical Optics",

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T1 - Pulse-modulated second harmonic imaging microscope quantitatively demonstrates marked increase of collagen in tumor after chemotherapy

AU - Raja, Anju M.

AU - Xu, Shuoyu

AU - Sun, Wanxin

AU - Zhou, Jianbiao

AU - Tai, Dean C.S.

AU - Chen, Chien Shing

AU - Rajapakse, Jagath C.

AU - So, Peter T.C.

AU - Yu, Hanry

N1 - Funding Information: This work is supported in part by the Institute of Bioengineering and Nanotechnology, BMRC, A*STAR of Singapore, and grants from BMRC (R185-001-045-305), Ministry of Education (R-185-000-135-112), Janssen-Cilag (R-185-000-182-592), Singapore-MIT Alliance Computational and Systems Biology Flagship Project funding (C-382-603-004-001), SMART BioSyM and Mechanobiology Institute, Singapore to Yu. Raja and Zhou are NUS Research Scholars, and Xu is a SMA Research Scholar.

PY - 2010/9

Y1 - 2010/9

N2 - Pulse-modulated second harmonic imaging microscopes (PM-SHIMs) exhibit improved signal-to-noise ratio (SNR) over conventional SHIMs on sensitive imaging and quantification of weak collagen signals inside tissues. We quantify the spatial distribution of sparse collagen inside a xenograft model of human acute myeloid leukemia (AML) tumor specimens treated with a new drug against receptor tyrosine kinase (ABT-869), and observe a significant increase in collagen area percentage, collagen fiber length, fiber width, and fiber number after chemotherapy. This finding reveals new insights into tumor responses to chemotherapy and suggests caution in developing new drugs and therapeutic regimens against cancers.

AB - Pulse-modulated second harmonic imaging microscopes (PM-SHIMs) exhibit improved signal-to-noise ratio (SNR) over conventional SHIMs on sensitive imaging and quantification of weak collagen signals inside tissues. We quantify the spatial distribution of sparse collagen inside a xenograft model of human acute myeloid leukemia (AML) tumor specimens treated with a new drug against receptor tyrosine kinase (ABT-869), and observe a significant increase in collagen area percentage, collagen fiber length, fiber width, and fiber number after chemotherapy. This finding reveals new insights into tumor responses to chemotherapy and suggests caution in developing new drugs and therapeutic regimens against cancers.

KW - collagen modulation postchemotherapy

KW - drug resistance

KW - pulse modulation

KW - second harmonic generation imaging

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Pulse-modulated second harmonic imaging microscope quantitatively demonstrates marked increase of collagen in tumor after chemotherapy

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ASJC Scopus Subject Areas

Keywords

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