TY - GEN
T1 - Protection of minocycline on early brain injury after subarachnoid hemorrhage in rats
AU - Guo, Zong Duo
AU - Wu, Hai Tao
AU - Sun, Xiao Chuan
AU - Zhang, Xiao Dong
AU - Zhang, John H.
N1 - Part of the Acta Neurochirurgica Supplements book series (NEUROCHIRURGICA, volume 110/1) Minocycline has been shown to be neuroprotective in cerebral ischemia and in other models of brain injury. Our goal is to observe the protection of minocycline on EBI after SAH and the mechanism.
PY - 2011/1/1
Y1 - 2011/1/1
N2 - Minocycline has been shown to be neuroprotective in cerebral ischemia and in other models of brain injury. Our goal is to observe the protection of minocycline on EBI after SAH and the mechanism. 48 adult male SD rats were randomly divided into four groups: the sham-operated group, SAH group, vehicle group (SAH + normal sodium), and minocycline group (SAH + minocycline). The SAH model was induced by injecting 300 μl of autologous arterial blood into the prechiasmatic cistern. Expressions of MMP-9 in the hippocampus were examined at 24 h by western blot and zymography. Western blot and zymography showed that the expression of total and active MMP-9 increased dramatically at 24 h after SAH compared with that of the sham group (P < 0.01). The clinical assessments got a lower score than that of the sham-operated group. After treated with minocycline, the expression of MMP-9 decreased significantly (P < 0.01 vs. vehicle group), and the clinical assessments improved. We conclude that minocycline can protect EBI after SAH, which may be related to the mechanism of inhibiting the expression of MMP-9 in the hippocampus. © 2011 Springer-Verlag/Wien.
AB - Minocycline has been shown to be neuroprotective in cerebral ischemia and in other models of brain injury. Our goal is to observe the protection of minocycline on EBI after SAH and the mechanism. 48 adult male SD rats were randomly divided into four groups: the sham-operated group, SAH group, vehicle group (SAH + normal sodium), and minocycline group (SAH + minocycline). The SAH model was induced by injecting 300 μl of autologous arterial blood into the prechiasmatic cistern. Expressions of MMP-9 in the hippocampus were examined at 24 h by western blot and zymography. Western blot and zymography showed that the expression of total and active MMP-9 increased dramatically at 24 h after SAH compared with that of the sham group (P < 0.01). The clinical assessments got a lower score than that of the sham-operated group. After treated with minocycline, the expression of MMP-9 decreased significantly (P < 0.01 vs. vehicle group), and the clinical assessments improved. We conclude that minocycline can protect EBI after SAH, which may be related to the mechanism of inhibiting the expression of MMP-9 in the hippocampus. © 2011 Springer-Verlag/Wien.
KW - Early brain injury
KW - Matrix metalloproteinase 9
KW - Minocycline
KW - Subarachnoid hemorrhage
KW - Matrix Metalloproteinase 9/metabolism
KW - Rats
KW - Male
KW - Brain Injuries/etiology
KW - Rats, Sprague-Dawley
KW - Subarachnoid Hemorrhage/complications
KW - Animals
KW - Analysis of Variance
KW - Gene Expression Regulation, Enzymologic/drug effects
KW - Minocycline/pharmacology
KW - Hippocampus/enzymology
KW - Neurologic Examination/methods
KW - Disease Models, Animal
UR - https://www.scopus.com/pages/publications/85052609912
UR - https://www.scopus.com/pages/publications/85052609912#tab=citedBy
UR - https://www.mendeley.com/catalogue/4b9fccef-3c02-3850-880b-4eb254e524e5/
U2 - 10.1007/978-3-7091-0353-1_13
DO - 10.1007/978-3-7091-0353-1_13
M3 - Conference contribution
C2 - 21116918
SN - 9783709103524
SN - 978-3-7091-1658-6
T3 - Acta Neurochirurgica, Supplementum
SP - 71
EP - 74
BT - Early Brain Injury or Cerebral Vasospasm
PB - Springer Vienna
ER -