Oxyhemoglobin produces apoptosis and necrosis in cultured smooth muscle cells

Kotaro Ogihara; Kazuya Aoki; Alexander Y. Zubkov; John H. Zhang

doi:10.1016/S0006-8993(00)03120-6

Oxyhemoglobin produces apoptosis and necrosis in cultured smooth muscle cells

Kotaro Ogihara, Kazuya Aoki, Alexander Y. Zubkov, John H. Zhang

Research output: Contribution to journal › Article › peer-review

Abstract

Confluent rat aortic smooth muscle cells were treated with OxyHb in a concentration- and time-dependent manner. A high concentration of OxyHb (100 μM) within 24 h decreased cell density. DNA analysis showed a smear pattern characteristic of cell necrosis. Transmission electron microscopy demonstrated disintegration of the cell membrane and destruction of cell organelles. Western blotting using PARP antibody revealed that 116 kDa PARP was not cleaved to 85 kDa, an apoptosis-related fragment. On the contrary, a low concentration of OxyHb (10 μM) produced apoptotic cell death at 72 h that was supported by DNA analysis and TUNEL staining. These results demonstrated that a high level of OxyHb induced necrosis within 24 h and a low concentration of OxyHb produced apoptosis after 72 h in cultured smooth muscle cells. Morphological alterations induced by OxyHb might contribute to the vascular wall changes in the cerebral arteries following subarachnoid hemorrhage (SAH).

Original language	English
Pages (from-to)	89-97
Number of pages	9
Journal	Brain Research
Volume	889
Issue number	1-2
DOIs	https://doi.org/10.1016/S0006-8993(00)03120-6
State	Published - Jan 19 2001
Externally published	Yes

ASJC Scopus Subject Areas

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

Keywords

Apoptosis
Necrosis
Oxyhemoglobin
Smooth muscle
Vasospasm
In Situ Nick-End Labeling
Apoptosis/drug effects
Rats
Cell Survival/drug effects
Microscopy, Electron
Muscle, Smooth, Vascular/drug effects
Blotting, Western
Poly(ADP-ribose) Polymerases/immunology
DNA/analysis
Animals
Oxyhemoglobins/metabolism
Aorta, Thoracic/drug effects

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title = "Oxyhemoglobin produces apoptosis and necrosis in cultured smooth muscle cells",

abstract = "Confluent rat aortic smooth muscle cells were treated with OxyHb in a concentration- and time-dependent manner. A high concentration of OxyHb (100 μM) within 24 h decreased cell density. DNA analysis showed a smear pattern characteristic of cell necrosis. Transmission electron microscopy demonstrated disintegration of the cell membrane and destruction of cell organelles. Western blotting using PARP antibody revealed that 116 kDa PARP was not cleaved to 85 kDa, an apoptosis-related fragment. On the contrary, a low concentration of OxyHb (10 μM) produced apoptotic cell death at 72 h that was supported by DNA analysis and TUNEL staining. These results demonstrated that a high level of OxyHb induced necrosis within 24 h and a low concentration of OxyHb produced apoptosis after 72 h in cultured smooth muscle cells. Morphological alterations induced by OxyHb might contribute to the vascular wall changes in the cerebral arteries following subarachnoid hemorrhage (SAH).",

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author = "Kotaro Ogihara and Kazuya Aoki and Zubkov, {Alexander Y.} and Zhang, {John H.}",

note = "Funding Information: This work was supported in part by a grant-in-aid to J.H.Z. from the American Heart Association. The authors thank Mr Glenn Hoskins for the support in electron microscopy. Electron microscope (LEO 906) is supported by grants 1S10RR11321-01A1 from National Institutes of Health. The authors thank Dr Corinne Gajdusek and Dr Marc R. Mayberg for providing the cultured rat aortic smooth muscle cells.",

year = "2001",

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doi = "10.1016/S0006-8993(00)03120-6",

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AU - Aoki, Kazuya

AU - Zubkov, Alexander Y.

AU - Zhang, John H.

N1 - Funding Information: This work was supported in part by a grant-in-aid to J.H.Z. from the American Heart Association. The authors thank Mr Glenn Hoskins for the support in electron microscopy. Electron microscope (LEO 906) is supported by grants 1S10RR11321-01A1 from National Institutes of Health. The authors thank Dr Corinne Gajdusek and Dr Marc R. Mayberg for providing the cultured rat aortic smooth muscle cells.

PY - 2001/1/19

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N2 - Confluent rat aortic smooth muscle cells were treated with OxyHb in a concentration- and time-dependent manner. A high concentration of OxyHb (100 μM) within 24 h decreased cell density. DNA analysis showed a smear pattern characteristic of cell necrosis. Transmission electron microscopy demonstrated disintegration of the cell membrane and destruction of cell organelles. Western blotting using PARP antibody revealed that 116 kDa PARP was not cleaved to 85 kDa, an apoptosis-related fragment. On the contrary, a low concentration of OxyHb (10 μM) produced apoptotic cell death at 72 h that was supported by DNA analysis and TUNEL staining. These results demonstrated that a high level of OxyHb induced necrosis within 24 h and a low concentration of OxyHb produced apoptosis after 72 h in cultured smooth muscle cells. Morphological alterations induced by OxyHb might contribute to the vascular wall changes in the cerebral arteries following subarachnoid hemorrhage (SAH).

AB - Confluent rat aortic smooth muscle cells were treated with OxyHb in a concentration- and time-dependent manner. A high concentration of OxyHb (100 μM) within 24 h decreased cell density. DNA analysis showed a smear pattern characteristic of cell necrosis. Transmission electron microscopy demonstrated disintegration of the cell membrane and destruction of cell organelles. Western blotting using PARP antibody revealed that 116 kDa PARP was not cleaved to 85 kDa, an apoptosis-related fragment. On the contrary, a low concentration of OxyHb (10 μM) produced apoptotic cell death at 72 h that was supported by DNA analysis and TUNEL staining. These results demonstrated that a high level of OxyHb induced necrosis within 24 h and a low concentration of OxyHb produced apoptosis after 72 h in cultured smooth muscle cells. Morphological alterations induced by OxyHb might contribute to the vascular wall changes in the cerebral arteries following subarachnoid hemorrhage (SAH).

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KW - Poly(ADP-ribose) Polymerases/immunology

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KW - Oxyhemoglobins/metabolism

KW - Aorta, Thoracic/drug effects

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ER -

Oxyhemoglobin produces apoptosis and necrosis in cultured smooth muscle cells

Abstract

ASJC Scopus Subject Areas

Keywords

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