TY - JOUR
T1 - Organosulfur Compounds of Garlic Modulate Mutagenesis, Metabolism, and DNA Binding of Aflatoxin Bj
AU - Tadi, Padma P.
AU - Teel, Robert W.
AU - Lau, Benjamin H.S.
N1 - Funding Information:
This study was supported by the Chan Shun Research Fund for AIDS and Cancer (Chan Shun International Foundation, Burlingame, CA). Address reprint requests to Dr. B.H.S. Lau, Dept. of Microbiology, School of Medicine, Loma Linda University, Loma Linda, CA 92350.
PY - 1991/2/1
Y1 - 1991/2/1
N2 - The effects of two organosulfur compounds of garlic (ajoene and diallyl sulfide) and a crude garlic extract on aflatoxin B 2(AFB-induced mutagenesis were determined using rat liver 9,000 g supernatant (S-9) as the activation system and Salmonella typhimurium TA-100 as the tester strain. The effects of these compounds on AFB 2 binding to calf thymus DNA were also measured. Metabolites of AFB 2 were isolated and analyzed by reverse-phase high-performance liquid chromatography. All these compounds inhibited S-9-dependent mutagenesis induced by AFB 2. They also inhibited AFB 2 binding to DNA. A significant decrease in organosoluble metabolites of AFB 2 was observed with ajoene and garlic extract. An increase of glucuronide and glutathione conjugates was obtained with garlic extract. The results indicate that garlic compounds tested in this study are antimutagenic and, potentially, anticarcinogenic.
AB - The effects of two organosulfur compounds of garlic (ajoene and diallyl sulfide) and a crude garlic extract on aflatoxin B 2(AFB-induced mutagenesis were determined using rat liver 9,000 g supernatant (S-9) as the activation system and Salmonella typhimurium TA-100 as the tester strain. The effects of these compounds on AFB 2 binding to calf thymus DNA were also measured. Metabolites of AFB 2 were isolated and analyzed by reverse-phase high-performance liquid chromatography. All these compounds inhibited S-9-dependent mutagenesis induced by AFB 2. They also inhibited AFB 2 binding to DNA. A significant decrease in organosoluble metabolites of AFB 2 was observed with ajoene and garlic extract. An increase of glucuronide and glutathione conjugates was obtained with garlic extract. The results indicate that garlic compounds tested in this study are antimutagenic and, potentially, anticarcinogenic.
UR - https://www.scopus.com/pages/publications/0025761796
UR - https://www.scopus.com/pages/publications/0025761796#tab=citedBy
U2 - 10.1080/01635589109514116
DO - 10.1080/01635589109514116
M3 - Article
C2 - 1903884
SN - 0163-5581
VL - 15
SP - 87
EP - 95
JO - Nutrition and Cancer
JF - Nutrition and Cancer
IS - 2
ER -