TY - JOUR
T1 - Non-colony type monolayer culture of human embryonic stem cells
AU - Chen, Kevin G.
AU - Mallon, Barbara S.
AU - Hamilton, Rebecca S.
AU - Kozhich, Olga A.
AU - Park, Kyeyoon
AU - Hoeppner, Daniel J.
AU - Robey, Pamela G.
AU - McKay, Ronald D.G.
N1 - Funding Information:
The NIH Stem Cell Unit is supported by NIH funds. We thank Dr. Joshua Chenoweth for the discussion, Dr. Peter Andrews for providing antibodies described in Supplemental information , Dr. Guokai Chen for the BC1 line, and Dr. Tianmin Ivy Zhang and Dr. Qi Zheng (Applied StemCell Inc.) for conducting the teratoma assays.
PY - 2012/11
Y1 - 2012/11
N2 - Regenerative medicine, relying on human embryonic stem cell (hESC) technology, opens promising new avenues for therapy of many severe diseases. However, this approach is restricted by limited production of the desired cells due to the refractory properties of hESC growth in vitro. It is further hindered by insufficient control of cellular stress, growth rates, and heterogeneous cellular states under current culture conditions. In this study, we report a novel cell culture method based on a non- colony type monolayer (NCM) growth. Human ESCs under NCM remain pluripotent as determined by teratoma assays and sustain the potential to differentiate into three germ layers. This NCM culture has been shown to homogenize cellular states, precisely control growth rates, significantly increase cell production, and enhance hESC recovery from cryopreservation without compromising chromosomal integrity. This culture system is simple, robust, scalable, and suitable for high-throughput screening and drug discovery.
AB - Regenerative medicine, relying on human embryonic stem cell (hESC) technology, opens promising new avenues for therapy of many severe diseases. However, this approach is restricted by limited production of the desired cells due to the refractory properties of hESC growth in vitro. It is further hindered by insufficient control of cellular stress, growth rates, and heterogeneous cellular states under current culture conditions. In this study, we report a novel cell culture method based on a non- colony type monolayer (NCM) growth. Human ESCs under NCM remain pluripotent as determined by teratoma assays and sustain the potential to differentiate into three germ layers. This NCM culture has been shown to homogenize cellular states, precisely control growth rates, significantly increase cell production, and enhance hESC recovery from cryopreservation without compromising chromosomal integrity. This culture system is simple, robust, scalable, and suitable for high-throughput screening and drug discovery.
UR - https://www.scopus.com/pages/publications/84865049791
UR - https://www.scopus.com/pages/publications/84865049791#tab=citedBy
U2 - 10.1016/j.scr.2012.06.003
DO - 10.1016/j.scr.2012.06.003
M3 - Article
C2 - 22910561
SN - 1873-5061
VL - 9
SP - 237
EP - 248
JO - Stem Cell Research
JF - Stem Cell Research
IS - 3
ER -