TY - JOUR
T1 - Mouse chromosome 9 quantitative trait loci for soft tissue regeneration
T2 - Congenic analysis and fine mapping
AU - Yu, Hongrun
AU - Baylink, David J.
AU - Masinde, Godfred L.
AU - Li, Runzhi
AU - Nguyen, Bay
AU - Davidson, Heather M.
AU - Xu, Shizhong
AU - Mohan, Subburaman
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PY - 2007/11
Y1 - 2007/11
N2 - Development of gene therapies for wound healing will depend on the identification of the genes involved in wound healing and tissue regeneration. Previous quantitative trait loci (QTL) studies in mice using the ear punch model have shown that major QTL exist on chromosome (Chr) 9 for soft tissue regeneration. In this study, we have developed a congenic line that contains the Chr 9 QTL chromosomal region from super healer MRL/MpJ in the genomic background of poor-healing SJL/J. The phenotypic effect of this QTL was confirmed in male mice, where the congenic line has shown significant healing improvement over SJL. Fine mapping of the Chr 9 QTL region with 23 markers at an average distance of 4.2 Mb using a total of 1,564 MRL/MpJ × SJL/J F 2 mice revealed the presence of at least three QTL peaks, implying that three separate loci may contribute to the phenotypic effect of this QTL. Based on the 2-LOD intervals, the total QTL region was confined to a combined length of no more than 28.2 Mb. Application of a Bayesian shrinkage estimation indicated that a major locus was located in a region of just 1.3 Mb. © 2007 by the Wound Healing Society.
AB - Development of gene therapies for wound healing will depend on the identification of the genes involved in wound healing and tissue regeneration. Previous quantitative trait loci (QTL) studies in mice using the ear punch model have shown that major QTL exist on chromosome (Chr) 9 for soft tissue regeneration. In this study, we have developed a congenic line that contains the Chr 9 QTL chromosomal region from super healer MRL/MpJ in the genomic background of poor-healing SJL/J. The phenotypic effect of this QTL was confirmed in male mice, where the congenic line has shown significant healing improvement over SJL. Fine mapping of the Chr 9 QTL region with 23 markers at an average distance of 4.2 Mb using a total of 1,564 MRL/MpJ × SJL/J F 2 mice revealed the presence of at least three QTL peaks, implying that three separate loci may contribute to the phenotypic effect of this QTL. Based on the 2-LOD intervals, the total QTL region was confined to a combined length of no more than 28.2 Mb. Application of a Bayesian shrinkage estimation indicated that a major locus was located in a region of just 1.3 Mb. © 2007 by the Wound Healing Society.
KW - Quantitative Trait, Heritable
KW - Wound Healing/genetics
KW - Genotype
KW - Genetic Markers/genetics
KW - Phenotype
KW - Quantitative Trait Loci/genetics
KW - Animals
KW - Analysis of Variance
KW - Epistasis, Genetic
KW - Regeneration/genetics
KW - Mice
KW - Mice, Congenic
KW - Soft Tissue Injuries/therapy
KW - Mice, Inbred MRL lpr/genetics
KW - Crosses, Genetic
UR - https://www.scopus.com/pages/publications/36248937659
UR - https://www.scopus.com/pages/publications/36248937659#tab=citedBy
UR - https://www.mendeley.com/catalogue/c561cd6e-b340-3892-a25c-6eeb63cb3f16/
U2 - 10.1111/j.1524-475X.2007.00317.x
DO - 10.1111/j.1524-475X.2007.00317.x
M3 - Article
C2 - 18028142
SN - 1067-1927
VL - 15
SP - 922
EP - 927
JO - Wound Repair and Regeneration
JF - Wound Repair and Regeneration
IS - 6
ER -