Molecular response of leukemia HL-60 cells to genistein treatment, a proteomics study

Daohai Zhang; Yan Chin Tai; Ching Ho Stephen Wong; Lee Kian Tai; Evelyn Siew Chuan Koay; Chien Shing Chen

doi:10.1016/j.leukres.2006.02.026

Molecular response of leukemia HL-60 cells to genistein treatment, a proteomics study

Daohai Zhang, Yan Chin Tai, Ching Ho Stephen Wong, Lee Kian Tai, Evelyn Siew Chuan Koay, Chien Shing Chen

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Abstract

Genistein (GEN) is a natural protein tyrosine kinase inhibitor. We analyzed the molecular response of HL-60 cells to GEN treatment by gel-based proteomics approach. Fourteen differentially expressed proteins which are functionally involved in metabolism, cell signaling, RNA processing, cell proliferation and motility, and chaperones were identified. Both the dose- and time-dependent up-regulation of Hsp70 protein 8 and heterogeneous nuclear ribonucleoprotein (hnRNP) H1, and the down-regulation of Rab14, hnRNP C and stathmin-1 by GEN were verified by immunoblot analysis. Our novel findings provide insightful clues to the potential therapeutic targets for leukemia treatment in diverse tyrosine kinase-dependent cellular pathways.

Original language	English
Pages (from-to)	75-82
Number of pages	8
Journal	Leukemia Research
Volume	31
Issue number	1
DOIs	https://doi.org/10.1016/j.leukres.2006.02.026
State	Published - Jan 2007

ASJC Scopus Subject Areas

Hematology
Oncology
Cancer Research

Keywords

Cell cycle arrest
Genistein
Leukemia HL-60
Proteomics
Tyrosine kinase

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title = "Molecular response of leukemia HL-60 cells to genistein treatment, a proteomics study",

abstract = "Genistein (GEN) is a natural protein tyrosine kinase inhibitor. We analyzed the molecular response of HL-60 cells to GEN treatment by gel-based proteomics approach. Fourteen differentially expressed proteins which are functionally involved in metabolism, cell signaling, RNA processing, cell proliferation and motility, and chaperones were identified. Both the dose- and time-dependent up-regulation of Hsp70 protein 8 and heterogeneous nuclear ribonucleoprotein (hnRNP) H1, and the down-regulation of Rab14, hnRNP C and stathmin-1 by GEN were verified by immunoblot analysis. Our novel findings provide insightful clues to the potential therapeutic targets for leukemia treatment in diverse tyrosine kinase-dependent cellular pathways.",

keywords = "Cell cycle arrest, Genistein, Leukemia HL-60, Proteomics, Tyrosine kinase",

author = "Daohai Zhang and Tai, {Yan Chin} and Wong, {Ching Ho Stephen} and Tai, {Lee Kian} and Koay, {Evelyn Siew Chuan} and Chen, {Chien Shing}",

note = "Funding Information: This work was supported by the Singapore Cancer Syndicate Grant (TN31).",

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AU - Zhang, Daohai

AU - Tai, Yan Chin

AU - Wong, Ching Ho Stephen

AU - Tai, Lee Kian

AU - Koay, Evelyn Siew Chuan

AU - Chen, Chien Shing

N1 - Funding Information: This work was supported by the Singapore Cancer Syndicate Grant (TN31).

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N2 - Genistein (GEN) is a natural protein tyrosine kinase inhibitor. We analyzed the molecular response of HL-60 cells to GEN treatment by gel-based proteomics approach. Fourteen differentially expressed proteins which are functionally involved in metabolism, cell signaling, RNA processing, cell proliferation and motility, and chaperones were identified. Both the dose- and time-dependent up-regulation of Hsp70 protein 8 and heterogeneous nuclear ribonucleoprotein (hnRNP) H1, and the down-regulation of Rab14, hnRNP C and stathmin-1 by GEN were verified by immunoblot analysis. Our novel findings provide insightful clues to the potential therapeutic targets for leukemia treatment in diverse tyrosine kinase-dependent cellular pathways.

AB - Genistein (GEN) is a natural protein tyrosine kinase inhibitor. We analyzed the molecular response of HL-60 cells to GEN treatment by gel-based proteomics approach. Fourteen differentially expressed proteins which are functionally involved in metabolism, cell signaling, RNA processing, cell proliferation and motility, and chaperones were identified. Both the dose- and time-dependent up-regulation of Hsp70 protein 8 and heterogeneous nuclear ribonucleoprotein (hnRNP) H1, and the down-regulation of Rab14, hnRNP C and stathmin-1 by GEN were verified by immunoblot analysis. Our novel findings provide insightful clues to the potential therapeutic targets for leukemia treatment in diverse tyrosine kinase-dependent cellular pathways.

KW - Cell cycle arrest

KW - Genistein

KW - Leukemia HL-60

KW - Proteomics

KW - Tyrosine kinase

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Molecular response of leukemia HL-60 cells to genistein treatment, a proteomics study

Abstract

ASJC Scopus Subject Areas

Keywords

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