TY - JOUR
T1 - Modulation of chemiluminescence in a murine macrophage cell line by neuroendocrine hormones
AU - Tosk, Jeffrey M.
AU - Grim, Joseph R.
AU - Kinback, Kevin M.
AU - Jeffrey Sale, E.
AU - Bozzetti, Louis P.
AU - Douglas Will, A.
N1 - Funding Information:
Acknowledgements -- This research was supported in part by a grant from the National Science Foundation (BNS-9016469) to J.M.T. and in part by a grant-in-aid from the Society of Sigma Xi to J.M.T. We wish to thank Dr John Leonora for his many helpful discussions and Alisa Johnson for manuscript preparation.
PY - 1993/7
Y1 - 1993/7
N2 - This study determined the effects of neuropeptides and neuroendocrine hormones at the cellular level of the immune response using a murine macrophage cell line, J774, which exhibits a chemiluminescent oxidative burst acute with stimulation with zymosan. We report that the zymosan-triggered oxidative burst of J774 cells can be modulated by the opioid peptides β-endorphin β-END and dynorphin A (DYN) in a naloxone-reversible fashion. Norepinephrine (NE) also modulated chemiluminescence (CL) emission of J774 cells, with dose-dependent suppression of CL dependent upon co-incubation with γ-interferon (γ-INF). Without γ-INF co-incubation, NE shared with the opioid peptides β-END and DYN the ability to modulate oxidative burst, producing an inverted-U dose response. These data indicate the J774 cells may be useful for explaining some mechanisms through which the neuroendocrine system interacts with the immune system.
AB - This study determined the effects of neuropeptides and neuroendocrine hormones at the cellular level of the immune response using a murine macrophage cell line, J774, which exhibits a chemiluminescent oxidative burst acute with stimulation with zymosan. We report that the zymosan-triggered oxidative burst of J774 cells can be modulated by the opioid peptides β-endorphin β-END and dynorphin A (DYN) in a naloxone-reversible fashion. Norepinephrine (NE) also modulated chemiluminescence (CL) emission of J774 cells, with dose-dependent suppression of CL dependent upon co-incubation with γ-interferon (γ-INF). Without γ-INF co-incubation, NE shared with the opioid peptides β-END and DYN the ability to modulate oxidative burst, producing an inverted-U dose response. These data indicate the J774 cells may be useful for explaining some mechanisms through which the neuroendocrine system interacts with the immune system.
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U2 - 10.1016/0192-0561(93)90079-E
DO - 10.1016/0192-0561(93)90079-E
M3 - Article
C2 - 8104166
SN - 0192-0561
VL - 15
SP - 615
EP - 620
JO - International Journal of Immunopharmacology
JF - International Journal of Immunopharmacology
IS - 5
ER -