TY - GEN
T1 - Matrix metalloproteinase 9 inhibition reduces early brain injury in cortex after subarachnoid hemorrhage
AU - Guo, Zong Duo
AU - Zhang, Xiao Dong
AU - Wu, Hai Tao
AU - Lin, Bin
AU - Sun, Xiao Chuan
AU - Zhang, John H.
N1 - This study investigated the role of matrix metalloproteinase-9 (MMP-9) in early brain injury (EBI) after subarachnoid hemorrhage (SAH). Sprague-Dawley male rats (n=30) between 250 and 300g were used.
PY - 2011/1/1
Y1 - 2011/1/1
N2 - This study investigated the role of matrix metalloproteinase-9 (MMP-9) in early brain injury (EBI) after subarachnoid hemorrhage (SAH). Sprague-Dawley male rats (n=30) between 250 and 300g were used. SAH was produced by injecting autologous arterial blood into the prechiasmatic cistern. SB-3CT, a selective MMP-9 inhibitor, was injected intraperitoneally after SAH induction. MMP-9 protein expression was measured by western blot; laminin expression and neuronal cells in the cerebral cortex were studied by immunohistochemistry and TUNEL staining at 24h after SAH. MMP-9 expression was increased after SAH and decreased by SB-3CT inhibition at 24h after SAH (P < 0.01). Laminin, the substrate of MMP-9, was decreased at 24h after SAH, and SB-3CT prevented laminin degradation. The number of TUNEL-positive neurons in cerebral cortex was increased after SAH and decreased by SB-3CT (P<0.01). MMP-9 may be involved in EBI after SAH and inhibition of MMP-9 may reduce EBI in cerebral cortex.
AB - This study investigated the role of matrix metalloproteinase-9 (MMP-9) in early brain injury (EBI) after subarachnoid hemorrhage (SAH). Sprague-Dawley male rats (n=30) between 250 and 300g were used. SAH was produced by injecting autologous arterial blood into the prechiasmatic cistern. SB-3CT, a selective MMP-9 inhibitor, was injected intraperitoneally after SAH induction. MMP-9 protein expression was measured by western blot; laminin expression and neuronal cells in the cerebral cortex were studied by immunohistochemistry and TUNEL staining at 24h after SAH. MMP-9 expression was increased after SAH and decreased by SB-3CT inhibition at 24h after SAH (P < 0.01). Laminin, the substrate of MMP-9, was decreased at 24h after SAH, and SB-3CT prevented laminin degradation. The number of TUNEL-positive neurons in cerebral cortex was increased after SAH and decreased by SB-3CT (P<0.01). MMP-9 may be involved in EBI after SAH and inhibition of MMP-9 may reduce EBI in cerebral cortex.
KW - Cell death
KW - Early brain injury
KW - Matrix metalloproteinase-9
KW - Subarachnoid hemorrhage
KW - In Situ Nick-End Labeling
KW - Laminin/metabolism
KW - Down-Regulation/drug effects
KW - Matrix Metalloproteinase 9/metabolism
KW - Neurons
KW - Rats
KW - Male
KW - Sulfones/pharmacology
KW - Rats, Sprague-Dawley
KW - Enzyme Inhibitors/pharmacology
KW - Heterocyclic Compounds, 1-Ring/pharmacology
KW - Subarachnoid Hemorrhage/complications
KW - Brain Injuries/enzymology
KW - Animals
KW - Analysis of Variance
KW - Cerebral Cortex/drug effects
KW - Disease Models, Animal
UR - https://www.scopus.com/pages/publications/85052609321
UR - https://www.scopus.com/pages/publications/85052609321#tab=citedBy
UR - https://www.mendeley.com/catalogue/efe08711-870d-3850-91a6-b2fb5ce634db/
U2 - 10.1007/978-3-7091-0353-1_15
DO - 10.1007/978-3-7091-0353-1_15
M3 - Conference contribution
C2 - 21116920
SN - 9783709103524
SN - 978-3-7091-1658-6
T3 - Acta Neurochirurgica, Supplementum
SP - 81
EP - 84
BT - Early Brain Injury or Cerebral Vasospasm
PB - Springer Vienna
ER -