TY - JOUR
T1 - Magnetic resonance imaging as a monitor of changes in the irradiated rat brain
T2 - An aid in determining the time course of events in a histologic study
AU - Kennedy, Andrew S.
AU - Archambeau, John O.
AU - Archambeau, Marie Helen
AU - Holshouser, Barbara
AU - Thompson, Joseph
AU - Moyers, Michael
AU - Hinshaw, David
AU - Slater, James M.
N1 - Invest Radiol. 1995 Apr;30(4):214-20. Research Support, Non-U.S. Gov't
PY - 1995/4
Y1 - 1995/4
N2 - RATIONALE AND OBJECTIVES. Optimal dose schedules and total dose of ionizing radiation for human central nervous system malignancy are not known. An animal model has been developed for the investigation of rat central nervous system response to proton irradiation using magnetic resonance imaging (MRI). A clinical MRI device was used to monitor the response of the rat brain after irradiation as a possible indicator for histologic injury as a function of time and dose. METHODS. Single-dose fractions of protons were delivered to the left brain of 25 adult Spraque-Dawley rats. Tl- and T2-weighted images were obtained using a 1.5-T MRI device via a 12-cm diameter coil at 4- to 6-week intervals after irradiation. Coronal images were evaluated by visual inspection and relaxation maps comparing the control and irradiated hemispheres. Histologic review was conducted on all rats' brains after death. RESULTS. Proton irradiation was delivered successfully to only the left brain of the animals. Histologic review confirms the location and extent of tissue damage demonstrated on MRIs obtained in vivo. Statistically significant differences were seen in the T2-weightcd relaxation times in the Irradiated cerebral hemisphere compared with the unirradiated hemisphere. CONCLUSION. The proton hemi brain rat model can be used to test treatment schedules of irradiation for central nervous system response using MRI to noninvasively document early and late effects within the same animal.
AB - RATIONALE AND OBJECTIVES. Optimal dose schedules and total dose of ionizing radiation for human central nervous system malignancy are not known. An animal model has been developed for the investigation of rat central nervous system response to proton irradiation using magnetic resonance imaging (MRI). A clinical MRI device was used to monitor the response of the rat brain after irradiation as a possible indicator for histologic injury as a function of time and dose. METHODS. Single-dose fractions of protons were delivered to the left brain of 25 adult Spraque-Dawley rats. Tl- and T2-weighted images were obtained using a 1.5-T MRI device via a 12-cm diameter coil at 4- to 6-week intervals after irradiation. Coronal images were evaluated by visual inspection and relaxation maps comparing the control and irradiated hemispheres. Histologic review was conducted on all rats' brains after death. RESULTS. Proton irradiation was delivered successfully to only the left brain of the animals. Histologic review confirms the location and extent of tissue damage demonstrated on MRIs obtained in vivo. Statistically significant differences were seen in the T2-weightcd relaxation times in the Irradiated cerebral hemisphere compared with the unirradiated hemisphere. CONCLUSION. The proton hemi brain rat model can be used to test treatment schedules of irradiation for central nervous system response using MRI to noninvasively document early and late effects within the same animal.
KW - Magnetic resonance imaging
KW - Proton therapy
KW - Radiation histology
KW - Rat brain radiation model
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U2 - 10.1097/00004424-199504000-00003
DO - 10.1097/00004424-199504000-00003
M3 - Article
C2 - 7635670
SN - 0020-9996
VL - 30
SP - 214
EP - 220
JO - Investigative Radiology
JF - Investigative Radiology
IS - 4
ER -