Isolation of wound healing/regeneration genes using restrictive fragment differential display-PCR in MRL/MPJ and C57BL/6 mice

Godfred Masinde; Xinmin Li; David J. Baylink; Bay Nguyen; Subburaman Mohan

doi:10.1016/j.bbrc.2005.02.143

Isolation of wound healing/regeneration genes using restrictive fragment differential display-PCR in MRL/MPJ and C57BL/6 mice

Godfred Masinde, Xinmin Li, David J. Baylink, Bay Nguyen, Subburaman Mohan

Research output: Contribution to journal › Article › peer-review

Abstract

Wound healing in mammals can take several weeks to months and the process is always accompanied by scar formation. Wound healing mechanisms that mimic regeneration are not found in most mature mammalian tissues. However, the MRL/MPJ (MRL) mouse has the unique capacity to regenerate ear hole wound completely in less than a month. To identify genes involved in wound healing without a scar, we chose to use restriction fragment differential display-PCR to isolate genes differentially expressed in the MRL (good healer) mouse and the C57BL/6 (poor healer) mouse at different stages of wound healing. We identified 36 genes that were differentially expressed in the regenerating tissue of good and poor healer strains of which several genes are also genetically linked to wound healing and thus are potential candidate genes for scarless wound healing.

Original language	English
Pages (from-to)	117-122
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	330
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2005.02.143
State	Published - Apr 29 2005

ASJC Scopus Subject Areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Keywords

Gene expression
Mice
QTL
RFDD-PCR
Regeneration
Wound healing

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title = "Isolation of wound healing/regeneration genes using restrictive fragment differential display-PCR in MRL/MPJ and C57BL/6 mice",

abstract = "Wound healing in mammals can take several weeks to months and the process is always accompanied by scar formation. Wound healing mechanisms that mimic regeneration are not found in most mature mammalian tissues. However, the MRL/MPJ (MRL) mouse has the unique capacity to regenerate ear hole wound completely in less than a month. To identify genes involved in wound healing without a scar, we chose to use restriction fragment differential display-PCR to isolate genes differentially expressed in the MRL (good healer) mouse and the C57BL/6 (poor healer) mouse at different stages of wound healing. We identified 36 genes that were differentially expressed in the regenerating tissue of good and poor healer strains of which several genes are also genetically linked to wound healing and thus are potential candidate genes for scarless wound healing.",

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author = "Godfred Masinde and Xinmin Li and Baylink, {David J.} and Bay Nguyen and Subburaman Mohan",

note = "Funding Information: The research reported in this manuscript was supported by the Department of the Army Cooperative Agreement Number (DAMD 17-97-2-7016). The views and content of the information do not necessarily reflect the position or the policy of the government or NMTB, and that no official endorsement should be inferred. The authors thank Debleena Sinha, Anil Kapoor, Nia Wedhas, Heather Davidson, and Melanie Hamilton-Ulland for their excellent technical support, and Shelley Levtzow and Sean Belcher for secretarial help. All work was performed at the Jerry L. Pettis VA Medical Research Facilities in Loma Linda, CA.",

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AU - Mohan, Subburaman

N1 - Funding Information: The research reported in this manuscript was supported by the Department of the Army Cooperative Agreement Number (DAMD 17-97-2-7016). The views and content of the information do not necessarily reflect the position or the policy of the government or NMTB, and that no official endorsement should be inferred. The authors thank Debleena Sinha, Anil Kapoor, Nia Wedhas, Heather Davidson, and Melanie Hamilton-Ulland for their excellent technical support, and Shelley Levtzow and Sean Belcher for secretarial help. All work was performed at the Jerry L. Pettis VA Medical Research Facilities in Loma Linda, CA.

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N2 - Wound healing in mammals can take several weeks to months and the process is always accompanied by scar formation. Wound healing mechanisms that mimic regeneration are not found in most mature mammalian tissues. However, the MRL/MPJ (MRL) mouse has the unique capacity to regenerate ear hole wound completely in less than a month. To identify genes involved in wound healing without a scar, we chose to use restriction fragment differential display-PCR to isolate genes differentially expressed in the MRL (good healer) mouse and the C57BL/6 (poor healer) mouse at different stages of wound healing. We identified 36 genes that were differentially expressed in the regenerating tissue of good and poor healer strains of which several genes are also genetically linked to wound healing and thus are potential candidate genes for scarless wound healing.

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KW - Regeneration

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Isolation of wound healing/regeneration genes using restrictive fragment differential display-PCR in MRL/MPJ and C57BL/6 mice

Abstract

ASJC Scopus Subject Areas

Keywords

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