IRE1α inhibition attenuates neuronal pyroptosis via miR-125/NLRP1 pathway in a neonatal hypoxic-ischemic encephalopathy rat model

Juan Huang, Weitian Lu, Desislava Met Doycheva, Marcin Gamdzyk, Xiao Hu, Rui Liu, John H. Zhang, Jiping Tang

Research output: Contribution to journalArticlepeer-review

Abstract

BACKGROUND: Inhibition of inositol-requiring enzyme-1 alpha (IRE1α), one of the sensor signaling proteins associated with endoplasmic reticulum (ER) stress, has been shown to alleviate brain injury and improve neurological behavior in a neonatal hypoxic-ischemic encephalopathy (HIE) rat model. However, there is no information about the role of IRE1α inhibitor as well as its molecular mechanisms in preventing neuronal pyroptosis induced by NLRP1 (NOD-, LRR- and pyrin domain-containing 1) inflammasome. In the present study, we hypothesized that IRE1α can degrade microRNA-125-b-2-3p (miR-125-b-2-3p) and activate NLRP1/caspased-1 pathway, and subsequently promote neuronal pyroptosis in HIE rat model.

METHODS: Ten-day old unsexed rat pups were subjected to hypoxia-ischemia (HI) injury, and the inhibitor of IRE1α, STF083010, was administered intranasally at 1 h after HI induction. AntimiR-125 or NLRP1 activation CRISPR was administered by intracerebroventricular (i.c.v) injection at 24 h before HI induction. Immunofluorescence staining, western blot analysis, reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR), brain infarct volume measurement, neurological function tests, and Fluoro-Jade C staining were performed.

RESULTS: Endogenous phosphorylated IRE1α (p-IRE1α), NLRP1, cleaved caspase-1, interleukin-1β (IL-1β), and interleukin-18 (IL-18) were increased and miR-125-b-2-3p was decreased in HIE rat model. STF083010 administration significantly upregulated the expression of miR-125-b-2-3p, reduced the infarct volume, improved neurobehavioral outcomes and downregulated the protein expression of NLRP1, cleaved caspase-1, IL-1β and IL-18. The protective effects of STF083010 were reversed by antimiR-125 or NLRP1 activation CRISPR.

CONCLUSIONS: IRE1α inhibitor, STF083010, reduced neuronal pyroptosis at least in part via miR-125/NLRP1/caspase-1 signaling pathway after HI.

Original languageEnglish
Article number152
JournalJournal of Neuroinflammation
Volume17
Issue number1
DOIs
StatePublished - May 6 2020

ASJC Scopus Subject Areas

  • General Neuroscience
  • Immunology
  • Neurology
  • Cellular and Molecular Neuroscience

Keywords

  • Hypoxic-ischemic encephalopathy
  • IRE1α
  • Inflammasome
  • NLPR1
  • Pyroptosis
  • miR-125
  • Animals, Newborn
  • Endoribonucleases/antagonists & inhibitors
  • Rats
  • Thiophenes/pharmacology
  • Neurons/drug effects
  • Rats, Sprague-Dawley
  • Signal Transduction/drug effects
  • Pyroptosis/drug effects
  • Animals
  • Nerve Tissue Proteins/metabolism
  • Sulfonamides/pharmacology
  • Multienzyme Complexes/antagonists & inhibitors
  • Hypoxia-Ischemia, Brain/metabolism
  • Inflammasomes/drug effects
  • MicroRNAs/metabolism
  • Protein Serine-Threonine Kinases/antagonists & inhibitors
  • Disease Models, Animal

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