TY - JOUR
T1 - Interactions of growth factors present in bone matrix with bone cells
T2 - Effects on DNA synthesis and alkaline phosphatase
AU - Kasperk, Christian H.
AU - Wergedal, Jon E.
AU - Mohan, Subburaman
AU - Long, Dana L.
AU - William Latj, K. H.
AU - Baylink, David J.
N1 - Funding Information:
One of the authors (C.H.K.) is a recipient of a post- doctoral fellowship of the Deutsche Forschungsgemein-schaft Bonn, FRG. This work was supported by grants from the Deutsche Gesellschaft fur Zahn-Mund- und Kieferheilkunde Dusseldorf, West Germany, the state of Schleswig-Holstein (Kultusministerium), West Germany and by medical research grants of the Veterans Administration and the NU3 ( # AR31Oh2-07).
PY - 1990
Y1 - 1990
N2 - It has been shown that bone cells produce and secrete several growth factors (GFs) which are also found in the bone matrix. To investigate the role of these growth factors in bone cell metabolism, we compared the effects of different factors separately and in combination with respect to osteoblastic cell proliferation and differentiation. While basic fibroblast GF (FGF), transforming GF β1 (TGFβ and platelet-derived GF (PDGF) enhance DNA synthesis, they had the opposite effect on alkaline phosphatase (ALP) activity in cell extracts: FGF, TGFβ and PDGF inhibited cell ALP but strongly stimulated DNA synthesis. The IGFs had little effect on cell ALP but increased the release of ALP into the conditioned medium. In mitogenic tests of combinations of GFs, most had at least additive effects at low concentrations, and FGF, TGFβ and IGF2 produced synergistic effects. Evidence is presented for (1) the modulation of the effects of one GF by the action of other GF, (2) synergistic interactions between FGF, TGFβ and IGF2, and (3) a possible role for the observed interactions among GF for the mitogenic effect of human bone extract.
AB - It has been shown that bone cells produce and secrete several growth factors (GFs) which are also found in the bone matrix. To investigate the role of these growth factors in bone cell metabolism, we compared the effects of different factors separately and in combination with respect to osteoblastic cell proliferation and differentiation. While basic fibroblast GF (FGF), transforming GF β1 (TGFβ and platelet-derived GF (PDGF) enhance DNA synthesis, they had the opposite effect on alkaline phosphatase (ALP) activity in cell extracts: FGF, TGFβ and PDGF inhibited cell ALP but strongly stimulated DNA synthesis. The IGFs had little effect on cell ALP but increased the release of ALP into the conditioned medium. In mitogenic tests of combinations of GFs, most had at least additive effects at low concentrations, and FGF, TGFβ and IGF2 produced synergistic effects. Evidence is presented for (1) the modulation of the effects of one GF by the action of other GF, (2) synergistic interactions between FGF, TGFβ and IGF2, and (3) a possible role for the observed interactions among GF for the mitogenic effect of human bone extract.
KW - Additivism
KW - Bone
KW - Interactions
KW - Osteoblastic cells
KW - Synergism
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U2 - 10.3109/08977199009108277
DO - 10.3109/08977199009108277
M3 - Article
C2 - 2206557
SN - 0897-7194
VL - 3
SP - 147
EP - 158
JO - Growth Factors
JF - Growth Factors
IS - 2
ER -