Increased RhoA translocation in aorta of diabetic rats

Jiping Tang; Ikuyo Kusaka; Amber R. Massey; Shadon Rollins; John H. Zhang

doi:10.1111/j.1745-7254.2006.00325.x

Increased RhoA translocation in aorta of diabetic rats

Jiping Tang, Ikuyo Kusaka, Amber R. Massey, Shadon Rollins, John H. Zhang

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Abstract

Aim: To analyze RhoA expression and activation in the aorta of diabetic rats. Methods: Male SD rats (n=70) were divided into 2 groups: the diabetic group and the control group. Diabetes was induced by intravenous injection of streptozotocin (55 mg/kg). The Rats were studied 3 weeks after the induction of diabetes. Western blotting was used to measure the expression and activation of Rho. Results: Heart rate was measured 24 h/d; it decreased by 58±13 beats/min in the diabetic rats. Isometric tension showed that the contraction of diabetic aorta was significantly reduced compared with that of control aorta when stimulated by KCI and serotonin. The relaxation of the diabetic aorta was reduced when stimulated by acetylcholine. An enhanced RhoA translocation in the aortic tissues of diabetic rats was determined by a 90% increase in membrane-bound RhoA, indicating that the activation of RhoA is markedly increased in the diabetic aorta. Conclusion: Our data suggest that upregulated RhoA could be involved in the vascular dysfunction of diabetic rats. ©2006 CPS and SIMM.

Original language	English
Pages (from-to)	543-548
Number of pages	6
Journal	Acta Pharmacologica Sinica
Volume	27
Issue number	5
DOIs	https://doi.org/10.1111/j.1745-7254.2006.00325.x
State	Published - May 2006

ASJC Scopus Subject Areas

Pharmacology
Pharmacology (medical)

Keywords

Aorta
Contractility
Diabetes
RhoA
Heart Rate
Muscle Relaxation/drug effects
Up-Regulation
rhoA GTP-Binding Protein/genetics
Diabetes Mellitus, Experimental/chemically induced
Streptozocin
Acetylcholine/pharmacology
Aorta/metabolism
Rats
Male
Rats, Sprague-Dawley
Muscle, Smooth, Vascular/physiopathology
RNA, Messenger/genetics
Protein Transport
Animals
Potassium Chloride/pharmacology
Muscle Contraction/drug effects
Enzyme Activation

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title = "Increased RhoA translocation in aorta of diabetic rats",

abstract = "Aim: To analyze RhoA expression and activation in the aorta of diabetic rats. Methods: Male SD rats (n=70) were divided into 2 groups: the diabetic group and the control group. Diabetes was induced by intravenous injection of streptozotocin (55 mg/kg). The Rats were studied 3 weeks after the induction of diabetes. Western blotting was used to measure the expression and activation of Rho. Results: Heart rate was measured 24 h/d; it decreased by 58±13 beats/min in the diabetic rats. Isometric tension showed that the contraction of diabetic aorta was significantly reduced compared with that of control aorta when stimulated by KCI and serotonin. The relaxation of the diabetic aorta was reduced when stimulated by acetylcholine. An enhanced RhoA translocation in the aortic tissues of diabetic rats was determined by a 90% increase in membrane-bound RhoA, indicating that the activation of RhoA is markedly increased in the diabetic aorta. Conclusion: Our data suggest that upregulated RhoA could be involved in the vascular dysfunction of diabetic rats. {\textcopyright}2006 CPS and SIMM.",

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author = "Jiping Tang and Ikuyo Kusaka and Massey, {Amber R.} and Shadon Rollins and Zhang, {John H.}",

note = "Diabetes mellitus is a common metabolic disease world-wide, affecting approximately 150 million people in 2000. It is predicted to affect 220 million people by 2010[ 1 ]. Diabetes and its associated complications have become a public health problem of considerable magnitude. Cardiovascular disease causes most of the excess morbidity and mortality in diabetes mellitus[ 1 ].",

year = "2006",

month = may,

doi = "10.1111/j.1745-7254.2006.00325.x",

language = "English",

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pages = "543--548",

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T1 - Increased RhoA translocation in aorta of diabetic rats

AU - Tang, Jiping

AU - Kusaka, Ikuyo

AU - Massey, Amber R.

AU - Rollins, Shadon

AU - Zhang, John H.

N1 - Diabetes mellitus is a common metabolic disease world-wide, affecting approximately 150 million people in 2000. It is predicted to affect 220 million people by 2010[ 1 ]. Diabetes and its associated complications have become a public health problem of considerable magnitude. Cardiovascular disease causes most of the excess morbidity and mortality in diabetes mellitus[ 1 ].

PY - 2006/5

Y1 - 2006/5

N2 - Aim: To analyze RhoA expression and activation in the aorta of diabetic rats. Methods: Male SD rats (n=70) were divided into 2 groups: the diabetic group and the control group. Diabetes was induced by intravenous injection of streptozotocin (55 mg/kg). The Rats were studied 3 weeks after the induction of diabetes. Western blotting was used to measure the expression and activation of Rho. Results: Heart rate was measured 24 h/d; it decreased by 58±13 beats/min in the diabetic rats. Isometric tension showed that the contraction of diabetic aorta was significantly reduced compared with that of control aorta when stimulated by KCI and serotonin. The relaxation of the diabetic aorta was reduced when stimulated by acetylcholine. An enhanced RhoA translocation in the aortic tissues of diabetic rats was determined by a 90% increase in membrane-bound RhoA, indicating that the activation of RhoA is markedly increased in the diabetic aorta. Conclusion: Our data suggest that upregulated RhoA could be involved in the vascular dysfunction of diabetic rats. ©2006 CPS and SIMM.

AB - Aim: To analyze RhoA expression and activation in the aorta of diabetic rats. Methods: Male SD rats (n=70) were divided into 2 groups: the diabetic group and the control group. Diabetes was induced by intravenous injection of streptozotocin (55 mg/kg). The Rats were studied 3 weeks after the induction of diabetes. Western blotting was used to measure the expression and activation of Rho. Results: Heart rate was measured 24 h/d; it decreased by 58±13 beats/min in the diabetic rats. Isometric tension showed that the contraction of diabetic aorta was significantly reduced compared with that of control aorta when stimulated by KCI and serotonin. The relaxation of the diabetic aorta was reduced when stimulated by acetylcholine. An enhanced RhoA translocation in the aortic tissues of diabetic rats was determined by a 90% increase in membrane-bound RhoA, indicating that the activation of RhoA is markedly increased in the diabetic aorta. Conclusion: Our data suggest that upregulated RhoA could be involved in the vascular dysfunction of diabetic rats. ©2006 CPS and SIMM.

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SN - 1671-4083

VL - 27

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JO - Acta Pharmacologica Sinica

JF - Acta Pharmacologica Sinica

IS - 5

ER -

Increased RhoA translocation in aorta of diabetic rats

Abstract

ASJC Scopus Subject Areas

Keywords

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