TY - JOUR
T1 - Hyperbaric oxygen preconditioning reduces ischemia-reperfusion injury by inhibition of apoptosis via mitochondrial pathway in rat brain
AU - Li, J. S.
AU - Zhang, W.
AU - Kang, Z. M.
AU - Ding, S. J.
AU - Liu, W. W.
AU - Zhang, J. H.
AU - Guan, Y. T.
AU - Sun, X. J.
N1 - Funding Information:
This study was supported by the National Nature Science Foundation of China (No. 30,500,579 to Xue-Jun Sun) and Changzhou Health Foundation (WZ200712).
PY - 2009/4/10
Y1 - 2009/4/10
N2 - This study examined the hypothesis that apoptotic inhibition via mitochondrial pathway was involved in hyperbaric oxygen preconditioning (HBO-PC)-induced neuroprotection on ischemia-reperfusion injury in rat brain. Male Sprague-Dawley rats (250∼280 g, n=144) were divided into control, middle cerebral artery occlusion (MCAO) for 90 min, and HBO-PC plus MCAO groups. HBO-PC was conducted four times by giving 100% oxygen at 2.5 atm absolute (ATA), for 1 h at 12 h intervals for 2 days. At 24 h after the last HBO-PC, MCAO was performed and at 24 h after MCAO, neurological function, brain water content, infarct volume, and cell death were evaluated. Enzymatic activity of capase-3 and -9, and expression of cytochrome c, Bcl-2 and Bax proteins were performed in the samples from hippocampus, ischemic penumbra and core of the brain cortex, respectively. HBO-PC reduced brain edema, decreased infarction volume, and improved neurological recovery. HBO-PC reduced cytoplasm cytochrome c levels, decreased caspase enzyme activity, upregulated the ratio of Bcl-2 and Bax expression, and abated the apoptosis of ischemic tissue. HBO-PC protects brain tissues from ischemia-reperfusion injury by suppressing mitochondrial apoptotic pathways. © 2009 IBRO.
AB - This study examined the hypothesis that apoptotic inhibition via mitochondrial pathway was involved in hyperbaric oxygen preconditioning (HBO-PC)-induced neuroprotection on ischemia-reperfusion injury in rat brain. Male Sprague-Dawley rats (250∼280 g, n=144) were divided into control, middle cerebral artery occlusion (MCAO) for 90 min, and HBO-PC plus MCAO groups. HBO-PC was conducted four times by giving 100% oxygen at 2.5 atm absolute (ATA), for 1 h at 12 h intervals for 2 days. At 24 h after the last HBO-PC, MCAO was performed and at 24 h after MCAO, neurological function, brain water content, infarct volume, and cell death were evaluated. Enzymatic activity of capase-3 and -9, and expression of cytochrome c, Bcl-2 and Bax proteins were performed in the samples from hippocampus, ischemic penumbra and core of the brain cortex, respectively. HBO-PC reduced brain edema, decreased infarction volume, and improved neurological recovery. HBO-PC reduced cytoplasm cytochrome c levels, decreased caspase enzyme activity, upregulated the ratio of Bcl-2 and Bax expression, and abated the apoptosis of ischemic tissue. HBO-PC protects brain tissues from ischemia-reperfusion injury by suppressing mitochondrial apoptotic pathways. © 2009 IBRO.
KW - apoptosis
KW - hyperbaric oxygenation
KW - ischemic penumbra
KW - middle cerebral artery occlusion
KW - prevention
KW - Caspase 3/metabolism
KW - Male
KW - Cytochromes c/metabolism
KW - bcl-2-Associated X Protein/metabolism
KW - Brain/pathology
KW - Infarction, Middle Cerebral Artery/pathology
KW - Water/metabolism
KW - Apoptosis/physiology
KW - Mitochondria/physiology
KW - Hippocampus/pathology
KW - Cerebral Cortex/pathology
KW - Rats
KW - Random Allocation
KW - Motor Activity/physiology
KW - Rats, Sprague-Dawley
KW - Reperfusion Injury/pathology
KW - Animals
KW - Proto-Oncogene Proteins c-bcl-2/metabolism
KW - Caspase 9/metabolism
KW - Hyperbaric Oxygenation
UR - http://www.scopus.com/inward/record.url?scp=63349105632&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=63349105632&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/ed951739-e8fd-30c6-9b49-70bf16bba69b/
U2 - 10.1016/j.neuroscience.2009.01.011
DO - 10.1016/j.neuroscience.2009.01.011
M3 - Article
C2 - 19185051
SN - 0306-4522
VL - 159
SP - 1309
EP - 1315
JO - Neuroscience
JF - Neuroscience
IS - 4
ER -