Hyperbaric oxygen preconditioning attenuates hemorrhagic transformation through increasing PPARγ in hyperglycemic MCAO rats

Hetao Bian, Qin Hu, Xiping Liang, Di Chen, Bo Li, Jiping Tang, John H. Zhang

Research output: Contribution to journalArticlepeer-review

Abstract

Hyperbaric oxygen preconditioning (HBO-PC) has been demonstrated to attenuate hemorrhagic transformation (HT) after middle cerebral artery occlusion (MCAO) in hyperglycemic rats. However, the mechanisms remain to be illustrated. Recently, HBO-PC has been shown to activate peroxisome proliferator-activated receptor-gamma (PPARγ) by increasing 15d-PGJ2 in primary cultured neurons. We hypothesize that HBO-PC reduces HT by suppressing inflammation through increasing 15d-PGJ2 and activating PPARγ in hyperglycemic MCAO rats. HBO (2.5. ATA) was administered for 1. h daily for 5 consecutive days. The PPARγ inhibitor GW9662 was administered intraperitoneally to designated animals. Infarction volume, hemorrhage volume, neurological scores and mortality were analyzed. The levels of 15d-PGJ2, PPARγ, TNF-α and IL-1β, tight junction proteins as well as the activity of MMP-2 and MMP-9 were evaluated 24. h after MCAO. HBO-PC reduced HT, improved neurological function, down-regulated inflammatory molecules and inhibited the activation of MMP-9 by increasing 15d-PGJ2 and PPARγ at 24. h after MCAO. The results suggested that HBO-PC might be an alternative measure to decrease HT in ischemic stroke.

Original languageEnglish
Pages (from-to)22-29
Number of pages8
JournalExperimental Neurology
Volume265
DOIs
StatePublished - Mar 1 2015

ASJC Scopus Subject Areas

  • Neurology
  • Developmental Neuroscience

Keywords

  • Hemorrhagic transformation
  • Hyperbaric oxygen preconditioning
  • MCAO
  • PPARγ
  • Hyperbaric Oxygenation/methods
  • Rats
  • Male
  • Hyperglycemia/metabolism
  • Rats, Sprague-Dawley
  • PPAR gamma/biosynthesis
  • Animals
  • Cerebral Hemorrhage/metabolism
  • Infarction, Middle Cerebral Artery/metabolism
  • Ischemic Preconditioning/methods

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