TY - JOUR
T1 - Hyperbaric oxygen preconditioning attenuates hemorrhagic transformation through increasing PPARγ in hyperglycemic MCAO rats
AU - Bian, Hetao
AU - Hu, Qin
AU - Liang, Xiping
AU - Chen, Di
AU - Li, Bo
AU - Tang, Jiping
AU - Zhang, John H.
N1 - Publisher Copyright:
© 2014 Elsevier Inc.
PY - 2015/3/1
Y1 - 2015/3/1
N2 - Hyperbaric oxygen preconditioning (HBO-PC) has been demonstrated to attenuate hemorrhagic transformation (HT) after middle cerebral artery occlusion (MCAO) in hyperglycemic rats. However, the mechanisms remain to be illustrated. Recently, HBO-PC has been shown to activate peroxisome proliferator-activated receptor-gamma (PPARγ) by increasing 15d-PGJ2 in primary cultured neurons. We hypothesize that HBO-PC reduces HT by suppressing inflammation through increasing 15d-PGJ2 and activating PPARγ in hyperglycemic MCAO rats. HBO (2.5. ATA) was administered for 1. h daily for 5 consecutive days. The PPARγ inhibitor GW9662 was administered intraperitoneally to designated animals. Infarction volume, hemorrhage volume, neurological scores and mortality were analyzed. The levels of 15d-PGJ2, PPARγ, TNF-α and IL-1β, tight junction proteins as well as the activity of MMP-2 and MMP-9 were evaluated 24. h after MCAO. HBO-PC reduced HT, improved neurological function, down-regulated inflammatory molecules and inhibited the activation of MMP-9 by increasing 15d-PGJ2 and PPARγ at 24. h after MCAO. The results suggested that HBO-PC might be an alternative measure to decrease HT in ischemic stroke.
AB - Hyperbaric oxygen preconditioning (HBO-PC) has been demonstrated to attenuate hemorrhagic transformation (HT) after middle cerebral artery occlusion (MCAO) in hyperglycemic rats. However, the mechanisms remain to be illustrated. Recently, HBO-PC has been shown to activate peroxisome proliferator-activated receptor-gamma (PPARγ) by increasing 15d-PGJ2 in primary cultured neurons. We hypothesize that HBO-PC reduces HT by suppressing inflammation through increasing 15d-PGJ2 and activating PPARγ in hyperglycemic MCAO rats. HBO (2.5. ATA) was administered for 1. h daily for 5 consecutive days. The PPARγ inhibitor GW9662 was administered intraperitoneally to designated animals. Infarction volume, hemorrhage volume, neurological scores and mortality were analyzed. The levels of 15d-PGJ2, PPARγ, TNF-α and IL-1β, tight junction proteins as well as the activity of MMP-2 and MMP-9 were evaluated 24. h after MCAO. HBO-PC reduced HT, improved neurological function, down-regulated inflammatory molecules and inhibited the activation of MMP-9 by increasing 15d-PGJ2 and PPARγ at 24. h after MCAO. The results suggested that HBO-PC might be an alternative measure to decrease HT in ischemic stroke.
KW - Hemorrhagic transformation
KW - Hyperbaric oxygen preconditioning
KW - MCAO
KW - PPARγ
KW - Hyperbaric Oxygenation/methods
KW - Rats
KW - Male
KW - Hyperglycemia/metabolism
KW - Rats, Sprague-Dawley
KW - PPAR gamma/biosynthesis
KW - Animals
KW - Cerebral Hemorrhage/metabolism
KW - Infarction, Middle Cerebral Artery/metabolism
KW - Ischemic Preconditioning/methods
UR - http://www.scopus.com/inward/record.url?scp=84925259234&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84925259234&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/d890c6fe-13ab-3ce6-9a87-dd4a1e141aaa/
U2 - 10.1016/j.expneurol.2014.12.016
DO - 10.1016/j.expneurol.2014.12.016
M3 - Article
C2 - 25542160
SN - 0014-4886
VL - 265
SP - 22
EP - 29
JO - Experimental Neurology
JF - Experimental Neurology
ER -