TY - JOUR
T1 - Hydrogen-rich saline protects against spinal cord injury in rats
AU - Chen, Chengwen
AU - Chen, Qianbo
AU - Mao, Yanfei
AU - Xu, Shengming
AU - Xia, Chunyan
AU - Shi, Xueyin
AU - Zhang, John H.
AU - Yuan, Hongbin
AU - Sun, Xuejun
N1 - Funding Information:
Acknowledgments This study was supported by grants from the fund of National Nature Science Foundation of China (30772092 to Dr. Xueyin Shi).and Disaster Medial Foundation at the Second Military University (200805 to Dr. Hongbin Yuan).
PY - 2010/7
Y1 - 2010/7
N2 - In the present study, we examined the mechanisms of hydrogen-rich saline, a reported therapeutic antioxidant, in the treatment of acute spinal cord contusion injury. Male Sprague-Dawley rats were used to produce a standardized model of contuses spinal cord injury (125 kdyn force). Hydrogen-rich saline was injected intraperitoneally (5 ml/kg) immediately, and at 24 and 48 h after injury. All rats were sacrificed at 72 h after spinal cord injury (SCI). Apoptotic cell death, oxidative stress, inflammation, level of Brain derived neurotrophic factor (BDNF) were evaluated. In addition, locomotor behavior was assessed using the Basso, Beattice and Bresnahan (BBB) scale. We observed that administration of hydrogen-rich saline decreased the number of apoptotic cells, suppressed oxidative stress, and improved locomotor functions. Hydrogen-rich saline increased the release of BDNF. In conclusion, hydrogen-rich saline reduced acute spinal cord contusion injury, possibly by reduction of oxidative stress and elevation of BDNF. © 2010 Springer Science+Business Media, LLC.
AB - In the present study, we examined the mechanisms of hydrogen-rich saline, a reported therapeutic antioxidant, in the treatment of acute spinal cord contusion injury. Male Sprague-Dawley rats were used to produce a standardized model of contuses spinal cord injury (125 kdyn force). Hydrogen-rich saline was injected intraperitoneally (5 ml/kg) immediately, and at 24 and 48 h after injury. All rats were sacrificed at 72 h after spinal cord injury (SCI). Apoptotic cell death, oxidative stress, inflammation, level of Brain derived neurotrophic factor (BDNF) were evaluated. In addition, locomotor behavior was assessed using the Basso, Beattice and Bresnahan (BBB) scale. We observed that administration of hydrogen-rich saline decreased the number of apoptotic cells, suppressed oxidative stress, and improved locomotor functions. Hydrogen-rich saline increased the release of BDNF. In conclusion, hydrogen-rich saline reduced acute spinal cord contusion injury, possibly by reduction of oxidative stress and elevation of BDNF. © 2010 Springer Science+Business Media, LLC.
KW - BDNF
KW - Hydrogen-rich saline
KW - Neuroprotection
KW - Oxidative free radicals
KW - Spinal cord injury
KW - Antioxidants/therapeutic use
KW - Protein Carbonylation
KW - Malondialdehyde/metabolism
KW - Oxidative Stress
KW - Rats
KW - Spinal Cord/metabolism
KW - Hindlimb/physiopathology
KW - Male
KW - Spinal Cord Injuries/metabolism
KW - Rats, Sprague-Dawley
KW - Caspases/metabolism
KW - Brain-Derived Neurotrophic Factor/metabolism
KW - Animals
KW - Sodium Chloride/therapeutic use
KW - Cell Death
KW - Hydrogen/therapeutic use
KW - Peroxidase/metabolism
KW - Apoptosis
UR - http://www.scopus.com/inward/record.url?scp=77953229191&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77953229191&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/fce8e406-1fa1-36da-b463-923e134059c9/
U2 - 10.1007/s11064-010-0162-y
DO - 10.1007/s11064-010-0162-y
M3 - Article
C2 - 20354783
SN - 0364-3190
VL - 35
SP - 1111
EP - 1118
JO - Neurochemical Research
JF - Neurochemical Research
IS - 7
ER -