TY - JOUR
T1 - Human pluripotent stem cell culture
T2 - Considerations for maintenance, expansion, and therapeutics
AU - Chen, Kevin G.
AU - Mallon, Barbara S.
AU - McKay, Ronald D.G.
AU - Robey, Pamela G.
N1 - Funding Information:
This work was supported by the Intramural Research Program of the National Institutes of Health (NIH) at the National Institute of Neurological Disorders and Stroke. We thank Dr. Peter Zandstra, Dr. Kyeyoon Park, Dr. Daniel Hoeppner, and Ms. Rebecca Hamilton for discussion and suggestions. We thank Mr. George Leiman for editorial assistance.
PY - 2014/1/2
Y1 - 2014/1/2
N2 - Human pluripotent stem cells (hPSCs) provide powerful resources for application in regenerative medicine and pharmaceutical development. In the past decade, various methods have been developed for large-scale hPSC culture that rely on combined use of multiple growth components, including media containing various growth factors, extracellular matrices, 3D environmental cues, andmodes of multicellular association. In this Protocol Review, we dissect these growth components by comparing cell culture methods and identifying the benefits and pitfalls associated with each one. We further provide criteria, considerations, and suggestions to achieve optimal cell growth for hPSC expansion, differentiation, and use in future therapeutic applications.
AB - Human pluripotent stem cells (hPSCs) provide powerful resources for application in regenerative medicine and pharmaceutical development. In the past decade, various methods have been developed for large-scale hPSC culture that rely on combined use of multiple growth components, including media containing various growth factors, extracellular matrices, 3D environmental cues, andmodes of multicellular association. In this Protocol Review, we dissect these growth components by comparing cell culture methods and identifying the benefits and pitfalls associated with each one. We further provide criteria, considerations, and suggestions to achieve optimal cell growth for hPSC expansion, differentiation, and use in future therapeutic applications.
UR - http://www.scopus.com/inward/record.url?scp=84896836202&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84896836202&partnerID=8YFLogxK
U2 - 10.1016/j.stem.2013.12.005
DO - 10.1016/j.stem.2013.12.005
M3 - Review article
C2 - 24388173
SN - 1934-5909
VL - 14
SP - 13
EP - 26
JO - Cell Stem Cell
JF - Cell Stem Cell
IS - 1
ER -