Genetic evidence for an interaction of the UbiG O-methyltransferase with UbiX in Escherichia coli coenzyme Q biosynthesis

Melissa Gulmezian; Haitao Zhang; George T. Javor; Catherine F. Clarke

doi:10.1128/JB.00668-06

Genetic evidence for an interaction of the UbiG O-methyltransferase with UbiX in Escherichia coli coenzyme Q biosynthesis

Melissa Gulmezian
, Haitao Zhang
, George T. Javor
, Catherine F. Clarke

Biochemistry and Microbiology

Research output: Contribution to journal › Article › peer-review

Abstract

IS16 is a thiol-sensitive, Q-deficient mutant strain of Escherichia coli. Here, we show that IS16 harbors a mutation in the ubiG gene encoding a methyltransferase required for two O-methylation steps of Q biosynthesis. Complementation of IS16 with either ubiG or ubiX_K-12 reverses this phenotype, suggesting that UbiX may interact with UbiG.

Original language	English
Pages (from-to)	6435-6439
Number of pages	5
Journal	Journal of Bacteriology
Volume	188
Issue number	17
DOIs	https://doi.org/10.1128/JB.00668-06
State	Published - Sep 2006

ASJC Scopus Subject Areas

Microbiology
Molecular Biology

Access to Document

10.1128/JB.00668-06

OpenUrl availability

Full text

Cite this

@article{d7721fb9b8cf423986ad73bb7e652511,

title = "Genetic evidence for an interaction of the UbiG O-methyltransferase with UbiX in Escherichia coli coenzyme Q biosynthesis",

abstract = "IS16 is a thiol-sensitive, Q-deficient mutant strain of Escherichia coli. Here, we show that IS16 harbors a mutation in the ubiG gene encoding a methyltransferase required for two O-methylation steps of Q biosynthesis. Complementation of IS16 with either ubiG or ubiXK-12 reverses this phenotype, suggesting that UbiX may interact with UbiG.",

author = "Melissa Gulmezian and Haitao Zhang and Javor, \{George T.\} and Clarke, \{Catherine F.\}",

year = "2006",

month = sep,

doi = "10.1128/JB.00668-06",

language = "English",

volume = "188",

pages = "6435--6439",

journal = "Journal of Bacteriology",

issn = "0021-9193",

number = "17",

}

TY - JOUR

T1 - Genetic evidence for an interaction of the UbiG O-methyltransferase with UbiX in Escherichia coli coenzyme Q biosynthesis

AU - Gulmezian, Melissa

AU - Zhang, Haitao

AU - Javor, George T.

AU - Clarke, Catherine F.

PY - 2006/9

Y1 - 2006/9

N2 - IS16 is a thiol-sensitive, Q-deficient mutant strain of Escherichia coli. Here, we show that IS16 harbors a mutation in the ubiG gene encoding a methyltransferase required for two O-methylation steps of Q biosynthesis. Complementation of IS16 with either ubiG or ubiXK-12 reverses this phenotype, suggesting that UbiX may interact with UbiG.

AB - IS16 is a thiol-sensitive, Q-deficient mutant strain of Escherichia coli. Here, we show that IS16 harbors a mutation in the ubiG gene encoding a methyltransferase required for two O-methylation steps of Q biosynthesis. Complementation of IS16 with either ubiG or ubiXK-12 reverses this phenotype, suggesting that UbiX may interact with UbiG.

UR - https://www.scopus.com/pages/publications/33749013854

UR - https://www.scopus.com/pages/publications/33749013854#tab=citedBy

U2 - 10.1128/JB.00668-06

DO - 10.1128/JB.00668-06

M3 - Article

C2 - 16923914

SN - 0021-9193

VL - 188

SP - 6435

EP - 6439

JO - Journal of Bacteriology

JF - Journal of Bacteriology

IS - 17

ER -

Genetic evidence for an interaction of the UbiG O-methyltransferase with UbiX in Escherichia coli coenzyme Q biosynthesis

Abstract

ASJC Scopus Subject Areas

Access to Document

OpenUrl availability

Other files and links

Cite this