Establishing a Simple and Rapid Comet Assay of Archived Sperm Cell to Assess DNA Fragmentation in the Assisted Reproduction Technologies Laboratory

TY - JOUR

T1 - Establishing a Simple and Rapid Comet Assay of Archived Sperm Cell to Assess DNA Fragmentation in the Assisted Reproduction Technologies Laboratory

AU - Chan, P.J

AU - Corselli, J.U

AU - Patton, W.C

AU - Jacobson, J.D

AU - King, A

N1 - Objectives: Traditional comet assays have been based on initially living cells suspended in a mini-agarose gel. The present study involved the novel use of dried fixed sperm smears overlaid with a mini-agarose gel layer. The null hypothesis was that sperm fertilizing capacity was independent of sperm DNA integrity.

PY - 2000/9/1

Y1 - 2000/9/1

UR - https://www.fertstert.org/article/S0015-0282(00)01079-7/fulltext

U2 - 10.1016/S0015-0282(00)01079-7

DO - 10.1016/S0015-0282(00)01079-7

M3 - Meeting abstract

VL - 74

JO - Fertility and Sterility

JF - Fertility and Sterility

IS - S1

ER -