Establishing a Simple and Rapid Comet Assay of Archived Sperm Cell to Assess DNA Fragmentation in the Assisted Reproduction Technologies Laboratory

P.J Chan; J.U Corselli; W.C Patton; J.D Jacobson; A King; Philip J. Chan

doi:10.1016/S0015-0282(00)01079-7

Establishing a Simple and Rapid Comet Assay of Archived Sperm Cell to Assess DNA Fragmentation in the Assisted Reproduction Technologies Laboratory

P.J Chan, J.U Corselli, W.C Patton, J.D Jacobson, A King, Philip J. Chan

Research output: Contribution to journal › Article › peer-review

Original language	American English
Journal	Fertility and Sterility
Volume	74
DOIs	https://doi.org/10.1016/S0015-0282(00)01079-7
State	Published - Sep 1 2000

Disciplines

Biochemistry
Internal Medicine
Medicine and Health Sciences
Endocrinology
Molecular Biology
Biology

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@article{4a10e11655ed4f9e95a42e05f700660f,

title = "Establishing a Simple and Rapid Comet Assay of Archived Sperm Cell to Assess DNA Fragmentation in the Assisted Reproduction Technologies Laboratory",

author = "P.J Chan and J.U Corselli and W.C Patton and J.D Jacobson and A King and Chan, {Philip J.}",

note = "Objectives: Traditional comet assays have been based on initially living cells suspended in a mini-agarose gel. The present study involved the novel use of dried fixed sperm smears overlaid with a mini-agarose gel layer. The null hypothesis was that sperm fertilizing capacity was independent of sperm DNA integrity.",

year = "2000",

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doi = "10.1016/S0015-0282(00)01079-7",

language = "American English",

volume = "74",

journal = "Fertility and Sterility",

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T1 - Establishing a Simple and Rapid Comet Assay of Archived Sperm Cell to Assess DNA Fragmentation in the Assisted Reproduction Technologies Laboratory

AU - Chan, P.J

AU - Corselli, J.U

AU - Patton, W.C

AU - Jacobson, J.D

AU - King, A

AU - Chan, Philip J.

N1 - Objectives: Traditional comet assays have been based on initially living cells suspended in a mini-agarose gel. The present study involved the novel use of dried fixed sperm smears overlaid with a mini-agarose gel layer. The null hypothesis was that sperm fertilizing capacity was independent of sperm DNA integrity.

PY - 2000/9/1

Y1 - 2000/9/1

UR - https://www.fertstert.org/article/S0015-0282(00)01079-7/fulltext

U2 - 10.1016/S0015-0282(00)01079-7

DO - 10.1016/S0015-0282(00)01079-7

M3 - Article

VL - 74

JO - Fertility and Sterility

JF - Fertility and Sterility

ER -

Establishing a Simple and Rapid Comet Assay of Archived Sperm Cell to Assess DNA Fragmentation in the Assisted Reproduction Technologies Laboratory

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