Abstract
This study focused on the effect of electrostimulation of fastigial nucleus on the expression of NgR and on axonal regeneration after focal cerebral ischemiareperfusion in rats. Cerebral ischemia and reperfusion was induced by nylon monofilament. Ninety-six male SD rats were randomly divided into sham group and ischemic insult groups at 12 hours, 24 hours, and 1 to 3 weeks after cerebral ischemia-reperfusion. Reverse transcriptionpolymerase chain reaction (RT-PCR) was used to determine the changes of NgR mRNA expression. Immunohistochemistry was used to detect the expression of NgR protein and the state of axonal regeneration. Fastigial nucleus stimulation was applied at 2 hours after ischemia for one hour. The results demonstrated that NgR mRNA and protein in the infarcted cortex and hippocampus were significantly increased (p
| Original language | American English |
|---|---|
| Pages (from-to) | 6999-7007 |
| Number of pages | 9 |
| Journal | Frontiers in Bioscience |
| Volume | 13 |
| Issue number | 18 |
| DOIs | |
| State | Published - May 1 2008 |
ASJC Scopus Subject Areas
- General Biochemistry,Genetics and Molecular Biology
- General Immunology and Microbiology
Keywords
- Axonal regeneration
- Electro-stimulation
- Fastigial nucleus
- Focal cerebral Ischemia
- NgR
- Nerve Regeneration/physiology
- Rats
- Male
- Electric Stimulation Therapy
- Cerebral Infarction/physiopathology
- Rats, Sprague-Dawley
- Brain Ischemia/physiopathology
- Animals
- Axons/physiology
- Reperfusion Injury/physiopathology
- Cerebellar Nuclei/physiopathology
Disciplines
- Anatomy