Effects of crude antigenic fractions of Leishmania major on natural killer cell cytotoxicity, interferon-γ and interleukin-4 secretion from peripheric blood lymphocytes of unexposed individuals

Aslihan Turhan; Saied Mirshahidi; A. Kevser Pişkin; Bahar Çitak; Turgut Imir

doi:10.1016/S0165-2478(96)02691-0

Effects of crude antigenic fractions of Leishmania major on natural killer cell cytotoxicity, interferon-γ and interleukin-4 secretion from peripheric blood lymphocytes of unexposed individuals

Aslihan Turhan, Saied Mirshahidi, A. Kevser Pişkin, Bahar Çitak, Turgut Imir

Research output: Contribution to journal › Article › peer-review

Abstract

The crude antigenic fraction (CAF) isolated from Leishmania major was fractionated into three subfractions by sodium dodecyl sulphate-polyacrylamide gel (SDS-PAGE). The effects of CAF and its subfractions on NK cell cytotoxicity is investigated by chromium release assay. These subfractions designated as fractions 1, 2 and 3 correspond to 97.4-66 kD, 66-45 kD and 29 kD and below respectively. Although both CAF and its subfractions have inhibited the cytotoxicity of natural killer (NK) cells, the effects of fractions 2 and 3 were more pronounced. The effect of the fractions on the Interferon-γ (IFN-γ) and Interleukin-4 (IL-4) secretion by peripheric blood lymphocytes was also analyzed. It was found that CAF and fraction 1 induce IFN-γ secretion while on the other hand IL-4 secretion was mostly suppressed by fraction 2. Therefore, further research is being executed which focuses on the effects of CAF, fractions 1 and 2 on macrophage effector functions.

Original language	English
Pages (from-to)	115-118
Number of pages	4
Journal	Immunology Letters
Volume	55
Issue number	2
DOIs	https://doi.org/10.1016/S0165-2478(96)02691-0
State	Published - Feb 1997

ASJC Scopus Subject Areas

Immunology and Allergy
Immunology

Keywords

Cytotoxicity
Interferon-γ
Interleukin-4
Leishmania major
Natural killer cells

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Effects of crude antigenic fractions of Leishmania major on natural killer cell cytotoxicity, interferon-γ and interleukin-4 secretion from peripheric blood lymphocytes of unexposed individuals. / Turhan, Aslihan; Mirshahidi, Saied; Kevser Pişkin, A. et al.
In: Immunology Letters, Vol. 55, No. 2, 02.1997, p. 115-118.

Research output: Contribution to journal › Article › peer-review

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abstract = "The crude antigenic fraction (CAF) isolated from Leishmania major was fractionated into three subfractions by sodium dodecyl sulphate-polyacrylamide gel (SDS-PAGE). The effects of CAF and its subfractions on NK cell cytotoxicity is investigated by chromium release assay. These subfractions designated as fractions 1, 2 and 3 correspond to 97.4-66 kD, 66-45 kD and 29 kD and below respectively. Although both CAF and its subfractions have inhibited the cytotoxicity of natural killer (NK) cells, the effects of fractions 2 and 3 were more pronounced. The effect of the fractions on the Interferon-γ (IFN-γ) and Interleukin-4 (IL-4) secretion by peripheric blood lymphocytes was also analyzed. It was found that CAF and fraction 1 induce IFN-γ secretion while on the other hand IL-4 secretion was mostly suppressed by fraction 2. Therefore, further research is being executed which focuses on the effects of CAF, fractions 1 and 2 on macrophage effector functions.",

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AU - Imir, Turgut

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Effects of crude antigenic fractions of Leishmania major on natural killer cell cytotoxicity, interferon-γ and interleukin-4 secretion from peripheric blood lymphocytes of unexposed individuals

Abstract

ASJC Scopus Subject Areas

Keywords

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