TY - JOUR
T1 - Effect of methylprednisolone on the production of neutrophil migration inhibition factor by T lymphocytes (NIF-T)
AU - Wong, Lawrence G.
AU - Colburn, Keith K.
AU - Kacena, Amelia
AU - Weisbart, Richard H.
N1 - Immunopharmacology. 1981 Jun;3(2):179-85. Research Support, Non-U.S. Gov't
PY - 1981/6
Y1 - 1981/6
N2 - Glucocorticoids may suppress cell-mediated immunity by inhibiting lymphocyte mediator production or reducing the responsivenes of target cells to these mediators. Our laboratory recently described a newly recognized T-lymphocyte mediator, neutrophil migration inhibition factor from T-lymphocytes (NIF-T). In this report we assessed the effect of glucocorticoids on NIF-T activity. Methylprednisolone (MP) at concentrations as low as 10-7M inhibited NIF-T activity from peripheral blood lymphocytes (PBL) in response to staphylococcal protein A (SPA) and concanavalin A (Con A). However, MP at concentrations as high as 10-4 M did not alter the responsiveness of neutrophils to NIF-T. Therefore, the effect of MP on NIF-T activity was due to inhibition of mediator production. The effect of MP on NIF-T production was reversible in 24 hours. This finding is consistent with the clinical observation that alternate day therapy does not suppress cell-mediated immunity. Serum taken from a patient as early as one hour after oral administration of 100 mg of prednisone inhibited NIF-T production in vitro; serum obtained at 48 hr after prednisone had no measurable effect on NIF-T activity. In addition, MP inhibited NIF-T production by previously activated lymphocytes.
AB - Glucocorticoids may suppress cell-mediated immunity by inhibiting lymphocyte mediator production or reducing the responsivenes of target cells to these mediators. Our laboratory recently described a newly recognized T-lymphocyte mediator, neutrophil migration inhibition factor from T-lymphocytes (NIF-T). In this report we assessed the effect of glucocorticoids on NIF-T activity. Methylprednisolone (MP) at concentrations as low as 10-7M inhibited NIF-T activity from peripheral blood lymphocytes (PBL) in response to staphylococcal protein A (SPA) and concanavalin A (Con A). However, MP at concentrations as high as 10-4 M did not alter the responsiveness of neutrophils to NIF-T. Therefore, the effect of MP on NIF-T activity was due to inhibition of mediator production. The effect of MP on NIF-T production was reversible in 24 hours. This finding is consistent with the clinical observation that alternate day therapy does not suppress cell-mediated immunity. Serum taken from a patient as early as one hour after oral administration of 100 mg of prednisone inhibited NIF-T production in vitro; serum obtained at 48 hr after prednisone had no measurable effect on NIF-T activity. In addition, MP inhibited NIF-T production by previously activated lymphocytes.
KW - Glucocorticoids
KW - Neutrophil migration inhibition factor from T-lymphocytes (NIF-T)
KW - cell- mediated immunity
UR - https://www.scopus.com/pages/publications/0019770718
UR - https://www.scopus.com/pages/publications/0019770718#tab=citedBy
U2 - 10.1016/0162-3109(81)90018-7
DO - 10.1016/0162-3109(81)90018-7
M3 - Article
C2 - 6972936
SN - 0162-3109
VL - 3
SP - 179
EP - 185
JO - Immunopharmacology
JF - Immunopharmacology
IS - 2
ER -