TY - JOUR
T1 - Docosahexaenoic Acid Therapy of Experimental Ischemic Stroke
AU - Belayev, Ludmila
AU - Khoutorova, Larissa
AU - Atkins, Kristal D.
AU - Eady, Tiffany N.
AU - Hong, Song
AU - Lu, Yan
AU - Obenaus, Andre
AU - Bazan, Nicolas G.
N1 - Funding Information:
Sources of Funding This investigation was supported by NIH, NINDS Grant R01 NS046741 (NGB) and NIH, NCCAM Grant RC2 AT005909 (NGB). Tiffany N. Eady is a recipient of the Ruth L. Kirschstein National Research Service Awards for Individual Predoctoral MD/PhD and Other Dual Doctoral Degree Fellows (NIH, NIA Grant F30 AG032841).
PY - 2011/3
Y1 - 2011/3
N2 - We examined the neuroprotective efficacy of docosahexaenoic acid (DHA), an omega-3 essential fatty acid family member, in acute ischemic stroke; studied the therapeutic window; and investigated whether DHA administration after an ischemic stroke is able to salvage the penumbra. In each series described below, SD rats underwent 2 h of middle cerebral artery occlusion (MCAo). In series 1, DHA or saline was administered i. v. at 3, 4, 5, or 6 h after stroke. In series 2, MRI was conducted on days 1, 3 and 7. In series 3, DHA or saline was administered at 3 h, and lipidomic analysis was conducted on day 3. Treatment with DHA significantly improved behavior and reduced total infarct volume by a mean of 40% when administered at 3 h, by 66% at 4 h, and by 59% at 5 h. Total lesion volumes computed from T2-weighted images were reduced in the DHA group at all time points. Lipidomic analysis showed that DHA treatment potentiates neuroprotectin D1 (NPD1) synthesis in the penumbra 3 days after MCAo. DHA administration provides neurobehavioral recovery, reduces brain infarction and edema, and activates NPD1 synthesis in the penumbra when administered up to 5 h after focal cerebral ischemia in rats. © 2010 The Author(s).
AB - We examined the neuroprotective efficacy of docosahexaenoic acid (DHA), an omega-3 essential fatty acid family member, in acute ischemic stroke; studied the therapeutic window; and investigated whether DHA administration after an ischemic stroke is able to salvage the penumbra. In each series described below, SD rats underwent 2 h of middle cerebral artery occlusion (MCAo). In series 1, DHA or saline was administered i. v. at 3, 4, 5, or 6 h after stroke. In series 2, MRI was conducted on days 1, 3 and 7. In series 3, DHA or saline was administered at 3 h, and lipidomic analysis was conducted on day 3. Treatment with DHA significantly improved behavior and reduced total infarct volume by a mean of 40% when administered at 3 h, by 66% at 4 h, and by 59% at 5 h. Total lesion volumes computed from T2-weighted images were reduced in the DHA group at all time points. Lipidomic analysis showed that DHA treatment potentiates neuroprotectin D1 (NPD1) synthesis in the penumbra 3 days after MCAo. DHA administration provides neurobehavioral recovery, reduces brain infarction and edema, and activates NPD1 synthesis in the penumbra when administered up to 5 h after focal cerebral ischemia in rats. © 2010 The Author(s).
KW - Animal models
KW - Focal ischemia
KW - Magnetic resonance imaging
KW - Neuroprotection
UR - https://www.scopus.com/pages/publications/79951508676
UR - https://www.scopus.com/pages/publications/79951508676#tab=citedBy
UR - https://www.mendeley.com/catalogue/059096c5-3c1f-3242-bd17-6e490515d8a9/
U2 - 10.1007/s12975-010-0046-0
DO - 10.1007/s12975-010-0046-0
M3 - Article
SN - 1868-4483
VL - 2
SP - 33
EP - 41
JO - Translational Stroke Research
JF - Translational Stroke Research
IS - 1
ER -