DNA/RNA-Based Next-Generation Sequencing Improves the Early Diagnosis and Management of Neoplastic Bile Duct Strictures: A 6-Year, Prospective, Multi-Institutional, Real-Time Study

Rohit Das; Jeffrey Kleinberger; Tarek Sawas; Abigail I. Wald; Robert Bubar; Harkirat Singh; Jennifer Chennat; Asif Khalid; Stephanie L. Romutis; Mordechai Rabinovitz; Anna Tavakkoli; Kimberly J. Fairley; Brian A. Boone; Savreet Sarkaria; John Nasr; Nikhil R. Thiruvengadam; Charles Gabbert; Sultan Mahmood; Ahmed A. Messallam; Mihajlo Gjeorgjievski; Markus Goldschmiedt; Shyam Thakkar; Thomas Tielleman; Mohannad Dugum; Jon Reich; Shailendra Singh; Randall E. Brand; Kevin McGrath; Kenneth Fasanella; Anne Marie Lennon; Rekha Reddy; Roshan Shrestha; Daniel J. Ellis; Sara A. Singhi; Terrence Lee; Peter S. Kay; Ryan D. Lee; Tiffany Y. Chua; Ibrahim Nassour; James L. Buxbaum; Joanna K. Law; Yasser Bhat; Prija A. Jamidar; Emily R. Jonica; Jeffrey J. Easler; Wasseem Skef; Hendrikus Dutch Vanderveldt; Anil K. Dasyam; David A. Geller; Amer H. Zureikat; Herbert J. Zeh; Patricio M. Polanco; J. Wallis Marsh; Melissa Hogg; Kenneth K. Lee; James F. Pingpank; M. Haroon A. Choudry; Abhinav Humar; Carl Schmidt; John Mansour; Geoffrey Nunns; Melanie Ongchin; Alessandro Paniccia; Sigfred Lajara; Sara E. Monaco; N. Paul Ohori; Liron Pantanowitz; James M. Mitchell; Katelyn Smith; Nuha Shaker; Anju H. Singhi; Dennis Hsu; Anwaar Saeed; Ibrahim H. Sahin; Janie Zhang; Vikram Gorantla; John Rhee; Marina N. Nikiforova; Nisa Kubiliun; Adam Slivka; Aatur D. Singhi

doi:10.1053/j.gastro.2026.02.040

DNA/RNA-Based Next-Generation Sequencing Improves the Early Diagnosis and Management of Neoplastic Bile Duct Strictures: A 6-Year, Prospective, Multi-Institutional, Real-Time Study

Rohit Das
, Jeffrey Kleinberger
, Tarek Sawas
, Abigail I. Wald
, Robert Bubar
, Harkirat Singh
, Jennifer Chennat
, Asif Khalid
, Stephanie L. Romutis
, Mordechai Rabinovitz
, Anna Tavakkoli
, Kimberly J. Fairley
, Brian A. Boone
, Savreet Sarkaria
, John Nasr
, Nikhil R. Thiruvengadam
, Charles Gabbert
, Sultan Mahmood
, Ahmed A. Messallam
, Mihajlo Gjeorgjievski

Markus Goldschmiedt, Shyam Thakkar, Thomas Tielleman, Mohannad Dugum, Jon Reich, Shailendra Singh, Randall E. Brand, Kevin McGrath, Kenneth Fasanella, Anne Marie Lennon, Rekha Reddy, Roshan Shrestha, Daniel J. Ellis, Sara A. Singhi, Terrence Lee, Peter S. Kay, Ryan D. Lee, Tiffany Y. Chua, Ibrahim Nassour, James L. Buxbaum, Joanna K. Law, Yasser Bhat, Prija A. Jamidar, Emily R. Jonica, Jeffrey J. Easler, Wasseem Skef, Hendrikus Dutch Vanderveldt, Anil K. Dasyam, David A. Geller, Amer H. Zureikat, Herbert J. Zeh, Patricio M. Polanco, J. Wallis Marsh, Melissa Hogg, Kenneth K. Lee, James F. Pingpank, M. Haroon A. Choudry, Abhinav Humar, Carl Schmidt, John Mansour, Geoffrey Nunns, Melanie Ongchin, Alessandro Paniccia, Sigfred Lajara, Sara E. Monaco, N. Paul Ohori, Liron Pantanowitz, James M. Mitchell, Katelyn Smith, Nuha Shaker, Anju H. Singhi, Dennis Hsu, Anwaar Saeed, Ibrahim H. Sahin, Janie Zhang, Vikram Gorantla, John Rhee, Marina N. Nikiforova, Nisa Kubiliun, Adam Slivka, Aatur D. Singhi

Interdisciplinary Studies

Research output: Contribution to journal › Article › peer-review

Original language	English
Pages (from-to)	325-341
Number of pages	17
Journal	Gastroenterology
Volume	171
Issue number	2
DOIs	https://doi.org/10.1053/j.gastro.2026.02.040
State	Accepted/In press - 2026

ASJC Scopus Subject Areas

Hepatology
Gastroenterology

Keywords

Biliary Stricture
Cholangiocarcinoma
Early Diagnosis
Next-Generation Sequencing
Pancreatic Cancer

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10.1053/j.gastro.2026.02.040

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Das, R., Kleinberger, J., Sawas, T., Wald, A. I., Bubar, R., Singh, H., Chennat, J., Khalid, A., Romutis, S. L., Rabinovitz, M., Tavakkoli, A., Fairley, K. J., Boone, B. A., Sarkaria, S., Nasr, J., Thiruvengadam, N. R., Gabbert, C., Mahmood, S., Messallam, A. A., ... Singhi, A. D. (Accepted/In press). DNA/RNA-Based Next-Generation Sequencing Improves the Early Diagnosis and Management of Neoplastic Bile Duct Strictures: A 6-Year, Prospective, Multi-Institutional, Real-Time Study. Gastroenterology, 171(2), 325-341. https://doi.org/10.1053/j.gastro.2026.02.040

Das, R, Kleinberger, J, Sawas, T, Wald, AI, Bubar, R, Singh, H, Chennat, J, Khalid, A, Romutis, SL, Rabinovitz, M, Tavakkoli, A, Fairley, KJ, Boone, BA, Sarkaria, S, Nasr, J, Thiruvengadam, NR, Gabbert, C, Mahmood, S, Messallam, AA, Gjeorgjievski, M, Goldschmiedt, M, Thakkar, S, Tielleman, T, Dugum, M, Reich, J, Singh, S, Brand, RE, McGrath, K, Fasanella, K, Lennon, AM, Reddy, R, Shrestha, R, Ellis, DJ, Singhi, SA, Lee, T, Kay, PS, Lee, RD, Chua, TY, Nassour, I, Buxbaum, JL, Law, JK, Bhat, Y, Jamidar, PA, Jonica, ER, Easler, JJ, Skef, W, Vanderveldt, HD, Dasyam, AK, Geller, DA, Zureikat, AH, Zeh, HJ, Polanco, PM, Marsh, JW, Hogg, M, Lee, KK, Pingpank, JF, Choudry, MHA, Humar, A, Schmidt, C, Mansour, J, Nunns, G, Ongchin, M, Paniccia, A, Lajara, S, Monaco, SE, Ohori, NP, Pantanowitz, L, Mitchell, JM, Smith, K, Shaker, N, Singhi, AH, Hsu, D, Saeed, A, Sahin, IH, Zhang, J, Gorantla, V, Rhee, J, Nikiforova, MN, Kubiliun, N, Slivka, A & Singhi, AD 2026, 'DNA/RNA-Based Next-Generation Sequencing Improves the Early Diagnosis and Management of Neoplastic Bile Duct Strictures: A 6-Year, Prospective, Multi-Institutional, Real-Time Study', Gastroenterology, vol. 171, no. 2, pp. 325-341. https://doi.org/10.1053/j.gastro.2026.02.040

@article{c5ce03536a24417c92355aadd5a91e67,

title = "DNA/RNA-Based Next-Generation Sequencing Improves the Early Diagnosis and Management of Neoplastic Bile Duct Strictures: A 6-Year, Prospective, Multi-Institutional, Real-Time Study",

keywords = "Biliary Stricture, Cholangiocarcinoma, Early Diagnosis, Next-Generation Sequencing, Pancreatic Cancer",

author = "Rohit Das and Jeffrey Kleinberger and Tarek Sawas and Wald, \{Abigail I.\} and Robert Bubar and Harkirat Singh and Jennifer Chennat and Asif Khalid and Romutis, \{Stephanie L.\} and Mordechai Rabinovitz and Anna Tavakkoli and Fairley, \{Kimberly J.\} and Boone, \{Brian A.\} and Savreet Sarkaria and John Nasr and Thiruvengadam, \{Nikhil R.\} and Charles Gabbert and Sultan Mahmood and Messallam, \{Ahmed A.\} and Mihajlo Gjeorgjievski and Markus Goldschmiedt and Shyam Thakkar and Thomas Tielleman and Mohannad Dugum and Jon Reich and Shailendra Singh and Brand, \{Randall E.\} and Kevin McGrath and Kenneth Fasanella and Lennon, \{Anne Marie\} and Rekha Reddy and Roshan Shrestha and Ellis, \{Daniel J.\} and Singhi, \{Sara A.\} and Terrence Lee and Kay, \{Peter S.\} and Lee, \{Ryan D.\} and Chua, \{Tiffany Y.\} and Ibrahim Nassour and Buxbaum, \{James L.\} and Law, \{Joanna K.\} and Yasser Bhat and Jamidar, \{Prija A.\} and Jonica, \{Emily R.\} and Easler, \{Jeffrey J.\} and Wasseem Skef and Vanderveldt, \{Hendrikus Dutch\} and Dasyam, \{Anil K.\} and Geller, \{David A.\} and Zureikat, \{Amer H.\} and Zeh, \{Herbert J.\} and Polanco, \{Patricio M.\} and Marsh, \{J. Wallis\} and Melissa Hogg and Lee, \{Kenneth K.\} and Pingpank, \{James F.\} and Choudry, \{M. Haroon A.\} and Abhinav Humar and Carl Schmidt and John Mansour and Geoffrey Nunns and Melanie Ongchin and Alessandro Paniccia and Sigfred Lajara and Monaco, \{Sara E.\} and Ohori, \{N. Paul\} and Liron Pantanowitz and Mitchell, \{James M.\} and Katelyn Smith and Nuha Shaker and Singhi, \{Anju H.\} and Dennis Hsu and Anwaar Saeed and Sahin, \{Ibrahim H.\} and Janie Zhang and Vikram Gorantla and John Rhee and Nikiforova, \{Marina N.\} and Nisa Kubiliun and Adam Slivka and Singhi, \{Aatur D.\}",

note = "Publisher Copyright: {\textcopyright} 2026 The Author(s). Published by Elsevier Inc. on behalf of American Gastroenterological Association Institute. This is an open access article under the CC BY license. http://creativecommons.org/licenses/by/4.0/",

year = "2026",

doi = "10.1053/j.gastro.2026.02.040",

language = "English",

volume = "171",

pages = "325--341",

journal = "Gastroenterology",

issn = "0016-5085",

number = "2",

}

TY - JOUR

T1 - DNA/RNA-Based Next-Generation Sequencing Improves the Early Diagnosis and Management of Neoplastic Bile Duct Strictures

T2 - A 6-Year, Prospective, Multi-Institutional, Real-Time Study

AU - Das, Rohit

AU - Kleinberger, Jeffrey

AU - Sawas, Tarek

AU - Wald, Abigail I.

AU - Bubar, Robert

AU - Singh, Harkirat

AU - Chennat, Jennifer

AU - Khalid, Asif

AU - Romutis, Stephanie L.

AU - Rabinovitz, Mordechai

AU - Tavakkoli, Anna

AU - Fairley, Kimberly J.

AU - Boone, Brian A.

AU - Sarkaria, Savreet

AU - Nasr, John

AU - Thiruvengadam, Nikhil R.

AU - Gabbert, Charles

AU - Mahmood, Sultan

AU - Messallam, Ahmed A.

AU - Gjeorgjievski, Mihajlo

AU - Goldschmiedt, Markus

AU - Thakkar, Shyam

AU - Tielleman, Thomas

AU - Dugum, Mohannad

AU - Reich, Jon

AU - Singh, Shailendra

AU - Brand, Randall E.

AU - McGrath, Kevin

AU - Fasanella, Kenneth

AU - Lennon, Anne Marie

AU - Reddy, Rekha

AU - Shrestha, Roshan

AU - Ellis, Daniel J.

AU - Singhi, Sara A.

AU - Lee, Terrence

AU - Kay, Peter S.

AU - Lee, Ryan D.

AU - Chua, Tiffany Y.

AU - Nassour, Ibrahim

AU - Buxbaum, James L.

AU - Law, Joanna K.

AU - Bhat, Yasser

AU - Jamidar, Prija A.

AU - Jonica, Emily R.

AU - Easler, Jeffrey J.

AU - Skef, Wasseem

AU - Vanderveldt, Hendrikus Dutch

AU - Dasyam, Anil K.

AU - Geller, David A.

AU - Zureikat, Amer H.

AU - Zeh, Herbert J.

AU - Polanco, Patricio M.

AU - Marsh, J. Wallis

AU - Hogg, Melissa

AU - Lee, Kenneth K.

AU - Pingpank, James F.

AU - Choudry, M. Haroon A.

AU - Humar, Abhinav

AU - Schmidt, Carl

AU - Mansour, John

AU - Nunns, Geoffrey

AU - Ongchin, Melanie

AU - Paniccia, Alessandro

AU - Lajara, Sigfred

AU - Monaco, Sara E.

AU - Ohori, N. Paul

AU - Pantanowitz, Liron

AU - Mitchell, James M.

AU - Smith, Katelyn

AU - Shaker, Nuha

AU - Singhi, Anju H.

AU - Hsu, Dennis

AU - Saeed, Anwaar

AU - Sahin, Ibrahim H.

AU - Zhang, Janie

AU - Gorantla, Vikram

AU - Rhee, John

AU - Nikiforova, Marina N.

AU - Kubiliun, Nisa

AU - Slivka, Adam

AU - Singhi, Aatur D.

N1 - Publisher Copyright: © 2026 The Author(s). Published by Elsevier Inc. on behalf of American Gastroenterological Association Institute. This is an open access article under the CC BY license. http://creativecommons.org/licenses/by/4.0/

PY - 2026

Y1 - 2026

KW - Biliary Stricture

KW - Cholangiocarcinoma

KW - Early Diagnosis

KW - Next-Generation Sequencing

KW - Pancreatic Cancer

UR - https://www.scopus.com/pages/publications/105042623577

UR - https://www.scopus.com/pages/publications/105042623577#tab=citedBy

U2 - 10.1053/j.gastro.2026.02.040

DO - 10.1053/j.gastro.2026.02.040

M3 - Article

C2 - 41905432

AN - SCOPUS:105042623577

SN - 0016-5085

VL - 171

SP - 325

EP - 341

JO - Gastroenterology

JF - Gastroenterology

IS - 2

ER -

DNA/RNA-Based Next-Generation Sequencing Improves the Early Diagnosis and Management of Neoplastic Bile Duct Strictures: A 6-Year, Prospective, Multi-Institutional, Real-Time Study

ASJC Scopus Subject Areas

Keywords

Access to Document

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