TY - JOUR
T1 - DICER governs characteristics of glioma stem cells and the resulting tumors in xenograft mouse models of glioblastoma
AU - Mansouri, Sheila
AU - Singh, Sanjay
AU - Alamsahebpour, Amir
AU - Burrell, Kelly
AU - Li, Mira
AU - Karabork, Merve
AU - Ekinci, Can
AU - Koch, Elizabeth
AU - Solaroglu, Ihsan
AU - Chang, Jeffery T.
AU - Wouters, Bradly
AU - Aldape, Kenneth
AU - Zadeh, Gelareh
PY - 2016
Y1 - 2016
N2 - The RNAse III endonuclease DICER is a key regulator of microRNA (miRNA) biogenesis and is frequently decreased in a variety of malignancies. We characterized the role of DICER in glioblastoma (GB), specifically demonstrating its effects on the ability of glioma stem-like cells (GSCs) to form tumors in a mouse model of GB. DICER silencing in GSCs reduced their stem cell characteristics, while tumors arising from these cells were more aggressive, larger in volume, and displayed a higher proliferation index and lineage differentiation. The resulting tumors, however, were more sensitive to radiation treatment. Our results demonstrate that DICER silencing enhances the tumorigenic potential of GSCs, providing a platform for analysis of specific relevant miRNAs and development of potentially novel therapies against GB.
AB - The RNAse III endonuclease DICER is a key regulator of microRNA (miRNA) biogenesis and is frequently decreased in a variety of malignancies. We characterized the role of DICER in glioblastoma (GB), specifically demonstrating its effects on the ability of glioma stem-like cells (GSCs) to form tumors in a mouse model of GB. DICER silencing in GSCs reduced their stem cell characteristics, while tumors arising from these cells were more aggressive, larger in volume, and displayed a higher proliferation index and lineage differentiation. The resulting tumors, however, were more sensitive to radiation treatment. Our results demonstrate that DICER silencing enhances the tumorigenic potential of GSCs, providing a platform for analysis of specific relevant miRNAs and development of potentially novel therapies against GB.
KW - DICER
KW - GSC
KW - Glioblastoma (GB)
KW - Radiation resistance
KW - miRNA
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UR - http://www.scopus.com/inward/citedby.url?scp=84984903197&partnerID=8YFLogxK
U2 - 10.18632/oncotarget.10570
DO - 10.18632/oncotarget.10570
M3 - Article
C2 - 27421140
SN - 1949-2553
VL - 7
SP - 56431
EP - 56446
JO - Oncotarget
JF - Oncotarget
IS - 35
ER -