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Diaspirin crosslinked hemoglobin (dclhb) does not affect the anesthetic potency of isoflurane in rats

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Abstract

Hemoglobin solutions are being developed as oxygen carrying fluids for multiple clinical indications. Despite an early report of accentuation of ether anesthesia, the effect of hemoglobin on anesthetic potency has not been assessed. We assessed the effect of α-α diaspirin crosslinked hemoglobin (DCLHb) on the anesthetic requirement of isoflurane necessary to keep rats unresponsive to noxious stimuli (1.0 MAC [minimum alveolar concentration]). During isoflurane administration, each rat received one of the following fluid regimens: 44Hct/N-normal hematocrit and volume; 44Hct/H-8 0 ml of donor blood given as a hypervolemic bolus; 30Hct/H-5 0 ml of DCLHb given as an exchange transfusion and 8.0 ml as a hypervolemic bolus; or 16Hct/H-15.0 ml of DCLHb given as an exchange transfusion and 8.0 ml as a hypervolemic bolus. MAC was determined using a standard tail clamp technique. The isoflurane requirement to achieve 1.0 MAC was not different between the four groups. These results are consistent with a hypothesis that DCLHb does not change the anesthetic state.

Original languageEnglish
Pages (from-to)89-99
Number of pages11
JournalArtificial Cells, Blood Substitutes, and Biotechnology
Volume23
Issue number1
DOIs
StatePublished - Jan 1995

ASJC Scopus Subject Areas

  • Biotechnology
  • Biomedical Engineering

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