Construction Of A Novel Functional Bacterial Luciferase By Gene Fusion And Its Use As A Gene Marker In Low Light Video Image Analysis.

A. P. Escher; D. O’Kane; J. Lee; W. H. Langridge; A. A. Szalay

doi:10.1117/12.962702

Construction Of A Novel Functional Bacterial Luciferase By Gene Fusion And Its Use As A Gene Marker In Low Light Video Image Analysis.

A. P. Escher
, D. O’Kane
, J. Lee
, W. H. Langridge
, A. A. Szalay

Research output: Contribution to journal › Article › peer-review

Abstract

We have engineered a two subunit luciferase enzyme into a single functional polypeptide chain using site specific mutagenesis. We have determined, using low light video imaging, that the activity of this novel enzyme is similar to wild type luciferase when synthesized at low temperatures (15-20°C), but that it is sensitive in vivo to higher temperatures. We have used the gene encoding the monomeric bacterial luciferase as a gene marker in prokaryotic and eukaryotic organisms. Combined with low light video image analysis, it is a practical and powerful tool for quantitatively monitoring gene expression in vivo.

Original language	English
Pages (from-to)	230-235
Number of pages	6
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	1161
DOIs	https://doi.org/10.1117/12.962702
State	Published - Dec 22 1989
Externally published	Yes

ASJC Scopus Subject Areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

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AU - Langridge, W. H.

AU - Szalay, A. A.

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Construction Of A Novel Functional Bacterial Luciferase By Gene Fusion And Its Use As A Gene Marker In Low Light Video Image Analysis.

Abstract

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