Comparison of methods for specific depletion of ATP in Salmonella typhimurium

M. S. Johnson; B. L. Taylor

doi:10.1128/aem.59.10.3509-3512.1993

Comparison of methods for specific depletion of ATP in Salmonella typhimurium

M. S. Johnson, B. L. Taylor

Microbiology Division

Research output: Contribution to journal › Comment/debate › peer-review

Abstract

Three methods of ATP depletion in Salmonella typhimurium were compared. ATP concentrations were lowest after arsenate treatment. Arsenate or α- methylglucoside-plus-azide treatment nonspecifically lowered all nucleotide triphosphate levels. Histidine starvation in a hisF mutant was relatively specific for ATP depletion and therefore has potential in distinguishing ATP- dependent processes from processes dependent on other nucleotides.

Original language	English
Pages (from-to)	3509-3512
Number of pages	4
Journal	Applied and Environmental Microbiology
Volume	59
Issue number	10
DOIs	https://doi.org/10.1128/aem.59.10.3509-3512.1993
State	Published - 1993

ASJC Scopus Subject Areas

Biotechnology
Food Science
Applied Microbiology and Biotechnology
Ecology

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abstract = "Three methods of ATP depletion in Salmonella typhimurium were compared. ATP concentrations were lowest after arsenate treatment. Arsenate or α- methylglucoside-plus-azide treatment nonspecifically lowered all nucleotide triphosphate levels. Histidine starvation in a hisF mutant was relatively specific for ATP depletion and therefore has potential in distinguishing ATP- dependent processes from processes dependent on other nucleotides.",

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AB - Three methods of ATP depletion in Salmonella typhimurium were compared. ATP concentrations were lowest after arsenate treatment. Arsenate or α- methylglucoside-plus-azide treatment nonspecifically lowered all nucleotide triphosphate levels. Histidine starvation in a hisF mutant was relatively specific for ATP depletion and therefore has potential in distinguishing ATP- dependent processes from processes dependent on other nucleotides.

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Comparison of methods for specific depletion of ATP in Salmonella typhimurium

Abstract

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