@article{685dd31809734812983fe1f5a309a19d,
title = "Comparison of Ellman's reagent with N-(1-pyrenyl)maleimide for the determination of free sulfhydryl groups in reduced cellobiohydrolase I from Trichoderma reesei",
keywords = "Cellobiohydrolase I, Ellman's reagent, N-(1-Pyrenyl)maleimide, Sulfhydryl determination",
author = "Jonathan Woodward and Jodi Tate and Herrmann, {Paul C.} and Evans, {Barbara R.}",
note = "Funding Information: The enzyme cellobiohydrolase I (CBH I) from Trichoderma reesei was treated with 5 mM dithiothreitol at different pH values in order to reduce some or all of its 12 disulfide bridges. A discrepancy was found in the number of free sulfhydryl (SH) groups generated upon the reduction of CBH I when they were measured using N-(1-pyrenyl)maleimide (PM) or Ellman's reagent, 5,5'-dithiobis(2-nitrobenzoic acid). For example, the number of SH mol generated/mol CBH I at pH 8.5 was determined to be 16 and < 1 when measured using PM or Ellman's reagent, respectively. The low value obtained with Correspondence address: J. Woodward, Chemical Technology Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6194, USA. 1 Oak Ridge Science and Engineering Research Semester (ORSERS) Student from North Dakota State University, Fargo, North Dakota. 20RSERS student from Andrews University, Berrien Springs, Michigan. 3 Managed by Martin Marietta Energy Systems, Inc., for the U.S. Department of Energy under contract DE-AC05-84OR21400. This article has been authored by a contractor of the U.S. Government under contract No. DE-AC05-84OR21400. Accordingly, the US Government retains a nonexclusive, royalty-free license to publish or reproduce the published form of this contribution, or allow others to do so, for US Government purposes.",
year = "1993",
month = may,
doi = "10.1016/0165-022X(93)90042-M",
language = "English",
volume = "26",
pages = "121--129",
journal = "Journal of Biochemical and Biophysical Methods",
issn = "0165-022X",
number = "2-3",
}