TY - JOUR
T1 - Comparison of distortion product otoacoustic emissions in 28 inbred strains of mice
AU - Martin, Glen K.
AU - Vazquez, Ana E.
AU - Jimenez, Ana M.
AU - Stagner, Barden B.
AU - Howard, MacKenzie A.
AU - Lonsbury-Martin, Brenda L.
N1 - Funding Information:
This work was supported the NIH (DC000613, DC003114, DC004476). The authors thank Denise L Koivisto and Claudia Candreia for their assistance in collecting the mouse DPOAE data, Michael Syka and Karolina Uttlova for assisting with the data basing of the data, and three anonymous reviewers for their helpful comments.
PY - 2007/12
Y1 - 2007/12
N2 - Cochlear function was evaluated in a longitudinal study of 28 inbred strains of mice at 3 and 5 mo of age using measures of distortion product otoacoustic emissions (DPOAEs) in response to a federal initiative to develop rapid mouse phenotyping methodologies. DP-grams at f2 frequencies ranging from 6.3 to 54.2 kHz were obtained in about 3 min/ear by eliciting 2f1 - f2 DPOAEs in 0.1-octave steps of f2 with primary tones at L1 = L2 = 55, 65, and 75 dB SPL. CBA/CaJ mice exhibited average levels of ∼26 dB SPL and this strain was selected as the normal reference strain against which the others were compared. Based upon the configurations of their DP-grams, the 28 mouse strains could be categorized into four distinct groups. That is, nine of the strains including the CBA were designated as the CBA-like group because these mice displayed robust DPOAE levels across frequency. In contrast, the remaining three groups all exhibited irregular DP-gram patterns. Specifically, eight of the remaining 19 strains showed a progressive high- to low-frequency reduction in DPOAE levels that was typical of age-related hearing loss (AHL) associated with mouse strains homozygous for the ahl allele and were labeled as AHL-like strains. Seven strains demonstrating relatively even patterns of reduced DPOAE levels across the frequency-test range were designated as Flat-loss strains. Finally, the remaining four strains exhibited no measurable DPOAEs at either 3 or 5 mo of age and thus were classified as Absent strains. Extending the f2 test frequencies up to ∼54 kHz led to the detection of very early-onset reductions in cochlear function in non-CBA-like groups so that all strains could be categorized by 3 mo of age. Predictably, the AHL-like strains showed more pronounced DPOAE losses at 5 mo than at 3 mo. A similar deterioration in DPOAE levels was not apparent for the Flat-loss strains. Both the AHL-like and Flat-loss strains showed considerably more variability in DPOAE levels than did the CBA-like strains. Together, these findings indicate that DP-grams adequately reveal both frequency-specific loss patterns and details of inbred strain variability.
AB - Cochlear function was evaluated in a longitudinal study of 28 inbred strains of mice at 3 and 5 mo of age using measures of distortion product otoacoustic emissions (DPOAEs) in response to a federal initiative to develop rapid mouse phenotyping methodologies. DP-grams at f2 frequencies ranging from 6.3 to 54.2 kHz were obtained in about 3 min/ear by eliciting 2f1 - f2 DPOAEs in 0.1-octave steps of f2 with primary tones at L1 = L2 = 55, 65, and 75 dB SPL. CBA/CaJ mice exhibited average levels of ∼26 dB SPL and this strain was selected as the normal reference strain against which the others were compared. Based upon the configurations of their DP-grams, the 28 mouse strains could be categorized into four distinct groups. That is, nine of the strains including the CBA were designated as the CBA-like group because these mice displayed robust DPOAE levels across frequency. In contrast, the remaining three groups all exhibited irregular DP-gram patterns. Specifically, eight of the remaining 19 strains showed a progressive high- to low-frequency reduction in DPOAE levels that was typical of age-related hearing loss (AHL) associated with mouse strains homozygous for the ahl allele and were labeled as AHL-like strains. Seven strains demonstrating relatively even patterns of reduced DPOAE levels across the frequency-test range were designated as Flat-loss strains. Finally, the remaining four strains exhibited no measurable DPOAEs at either 3 or 5 mo of age and thus were classified as Absent strains. Extending the f2 test frequencies up to ∼54 kHz led to the detection of very early-onset reductions in cochlear function in non-CBA-like groups so that all strains could be categorized by 3 mo of age. Predictably, the AHL-like strains showed more pronounced DPOAE losses at 5 mo than at 3 mo. A similar deterioration in DPOAE levels was not apparent for the Flat-loss strains. Both the AHL-like and Flat-loss strains showed considerably more variability in DPOAE levels than did the CBA-like strains. Together, these findings indicate that DP-grams adequately reveal both frequency-specific loss patterns and details of inbred strain variability.
KW - Age-related hearing loss
KW - Cochlea
KW - Distortion product otoacoustic emissions
KW - Inbred mouse strains
KW - Mouse hearing screening
KW - Outer hair cell
UR - https://www.scopus.com/pages/publications/36048969151
UR - https://www.scopus.com/pages/publications/36048969151#tab=citedBy
U2 - 10.1016/j.heares.2007.09.002
DO - 10.1016/j.heares.2007.09.002
M3 - Article
C2 - 17997239
SN - 0378-5955
VL - 234
SP - 59
EP - 72
JO - Hearing Research
JF - Hearing Research
IS - 1-2
ER -