TY - JOUR
T1 - Cloning and characterization of the C.elegans histidyl-tRNA synthetase gene
AU - Amaar, Yousef G.
AU - Baillie, David L.
N1 - Funding Information:
We would like to thank Dr R.Waterston for kindly providing the genomic mapping filter. We would like also to thank Dinar Suleman, Marcia Wakarchuk, and Steve Jones for their technical assistance, and Marco Mara for his critical review of the manuscript. This work has been supported by a grant to D.L.B. from the NSERC and a scholarship to Y.A. from the Libyan Secretariat of Scientific Research.
PY - 1993/9/11
Y1 - 1993/9/11
N2 - In this paper, we report the cloning and sequencing of the C.elegans histldyl-tRNA synthetase gene. The complete genomlc sequence, and most of the cDNA sequence, of this gene Is now determined. The gene size Including flanking and coding regions Is 2230 nucleotides long. Three small Introns(45 - 50 bp long) are found to Interrupt the open reading frame. The open reading frame translates to 523 amlno acids. This ptative protein sequence shows extensive homology with the human and yeast histldyl-tRNA the histldyltRNA synthetase gene Is a single copy gene. Hence, it Is very likely that It encodes both the cytoplasmlc and the mltochondrlal histldyl-tRNA synthetases. It is likely to be trans-spliced since it contains a trans-splice site In its 5' untranslated region.
AB - In this paper, we report the cloning and sequencing of the C.elegans histldyl-tRNA synthetase gene. The complete genomlc sequence, and most of the cDNA sequence, of this gene Is now determined. The gene size Including flanking and coding regions Is 2230 nucleotides long. Three small Introns(45 - 50 bp long) are found to Interrupt the open reading frame. The open reading frame translates to 523 amlno acids. This ptative protein sequence shows extensive homology with the human and yeast histldyl-tRNA the histldyltRNA synthetase gene Is a single copy gene. Hence, it Is very likely that It encodes both the cytoplasmlc and the mltochondrlal histldyl-tRNA synthetases. It is likely to be trans-spliced since it contains a trans-splice site In its 5' untranslated region.
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U2 - 10.1093/nar/21.18.4344
DO - 10.1093/nar/21.18.4344
M3 - Article
C2 - 8414990
SN - 0305-1048
VL - 21
SP - 4344
EP - 4347
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 18
ER -