Changes in mRNA expression of adenosine receptors in human chronic periodontitis.

Chun Xiao Sun; Nathan R. Wall; Nikola Angelov; Craig Ririe; Jung Wei Chen; Danilo S. Boskovic; Jeffrey M. Henkin

Changes in mRNA expression of adenosine receptors in human chronic periodontitis.

Chun Xiao Sun
, Nathan R. Wall
, Nikola Angelov
, Craig Ririe
, Jung Wei Chen
, Danilo S. Boskovic
, Jeffrey M. Henkin

Pediatric Dentistry

Research output: Contribution to journal › Article › peer-review

Abstract

OBJECTIVE: To elucidate the aetiology of periodontitis, this study focused on the adenosine receptor (AR) expression profiles (A1AR, A2AAR, A2BAR and A3AR) in periodontal diseased tissues.

METHODS: Adenosine receptor gene expression levels in human gingiva from 15 patients with healthy gingival tissues (control group) and 15 patients who exhibited severe chronic periodontitis (test group) were measured using quantitative reverse transcription-polymerase chain reaction (RT-PCR).

RESULTS: The mRNA expression pattern changed in human chronic periodontitis: the A1AR decreased 20%, A2AAR increased 2.5-fold, A2BAR increased 3.7-fold and A3AR decreased 70% as compared with that of healthy gingiva.

CONCLUSION: Inflammation of the gingival tissue is associated with (1) an unchanged expression of A1AR, (2) an increased expression of A2AAR and A2BAR, and (3) a decreased expression of A3AR. Logistic regression analysis indicated that the change in the expression patterns can be used to diagnose/predict periodontitis. This finding indicates that the adenosine receptor expression profile is changed in periodontitis with the potential for future clinical application.

Original language	American English
Pages (from-to)	113-120
Number of pages	8
Journal	The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA)
Volume	14
Issue number	2
State	Published - Dec 15 2011

ASJC Scopus Subject Areas

General Medicine

Keywords

Chronic Periodontitis/genetics
Alveolar Bone Loss/metabolism
Humans
Middle Aged
Periodontal Attachment Loss/metabolism
Male
Gingiva/metabolism
Receptor, Adenosine A2A/analysis
Reverse Transcriptase Polymerase Chain Reaction
Gingivitis/metabolism
Receptor, Adenosine A1/analysis
Young Adult
Receptors, Purinergic P1/analysis
Aged, 80 and over
Adult
Female
RNA, Messenger/analysis
Aged
Receptor, Adenosine A2B/analysis
Gingival Hemorrhage/metabolism
Real-Time Polymerase Chain Reaction
Receptor, Adenosine A3/analysis

Disciplines

Biology
Internal Medicine
Endocrinology

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https://www.quintessence-publishing.com/deu/en/article/851688

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Sun, C. X., Wall, N. R., Angelov, N., Ririe, C., Chen, J. W., Boskovic, D. S., & Henkin, J. M. (2011). Changes in mRNA expression of adenosine receptors in human chronic periodontitis. The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA), 14(2), 113-120. https://www.quintessence-publishing.com/deu/en/article/851688

Changes in mRNA expression of adenosine receptors in human chronic periodontitis. / Sun, Chun Xiao; Wall, Nathan R.; Angelov, Nikola et al.
In: The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA), Vol. 14, No. 2, 15.12.2011, p. 113-120.

Research output: Contribution to journal › Article › peer-review

Sun, CX, Wall, NR, Angelov, N, Ririe, C, Chen, JW , Boskovic, DS & Henkin, JM 2011, 'Changes in mRNA expression of adenosine receptors in human chronic periodontitis.', The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA), vol. 14, no. 2, pp. 113-120. <https://www.quintessence-publishing.com/deu/en/article/851688>

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abstract = "OBJECTIVE: To elucidate the aetiology of periodontitis, this study focused on the adenosine receptor (AR) expression profiles (A1AR, A2AAR, A2BAR and A3AR) in periodontal diseased tissues.METHODS: Adenosine receptor gene expression levels in human gingiva from 15 patients with healthy gingival tissues (control group) and 15 patients who exhibited severe chronic periodontitis (test group) were measured using quantitative reverse transcription-polymerase chain reaction (RT-PCR).RESULTS: The mRNA expression pattern changed in human chronic periodontitis: the A1AR decreased 20\%, A2AAR increased 2.5-fold, A2BAR increased 3.7-fold and A3AR decreased 70\% as compared with that of healthy gingiva.CONCLUSION: Inflammation of the gingival tissue is associated with (1) an unchanged expression of A1AR, (2) an increased expression of A2AAR and A2BAR, and (3) a decreased expression of A3AR. Logistic regression analysis indicated that the change in the expression patterns can be used to diagnose/predict periodontitis. This finding indicates that the adenosine receptor expression profile is changed in periodontitis with the potential for future clinical application.",

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author = "Sun, \{Chun Xiao\} and Wall, \{Nathan R.\} and Nikola Angelov and Craig Ririe and Chen, \{Jung Wei\} and Boskovic, \{Danilo S.\} and Henkin, \{Jeffrey M.\}",

note = "Chin J Dent Res. 2011;14(2):113-20. Comparative Study; Research Support, Non-U.S. Gov't",

year = "2011",

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day = "15",

language = "American English",

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T1 - Changes in mRNA expression of adenosine receptors in human chronic periodontitis.

AU - Sun, Chun Xiao

AU - Wall, Nathan R.

AU - Angelov, Nikola

AU - Ririe, Craig

AU - Chen, Jung Wei

AU - Boskovic, Danilo S.

AU - Henkin, Jeffrey M.

N1 - Chin J Dent Res. 2011;14(2):113-20. Comparative Study; Research Support, Non-U.S. Gov't

PY - 2011/12/15

Y1 - 2011/12/15

N2 - OBJECTIVE: To elucidate the aetiology of periodontitis, this study focused on the adenosine receptor (AR) expression profiles (A1AR, A2AAR, A2BAR and A3AR) in periodontal diseased tissues.METHODS: Adenosine receptor gene expression levels in human gingiva from 15 patients with healthy gingival tissues (control group) and 15 patients who exhibited severe chronic periodontitis (test group) were measured using quantitative reverse transcription-polymerase chain reaction (RT-PCR).RESULTS: The mRNA expression pattern changed in human chronic periodontitis: the A1AR decreased 20%, A2AAR increased 2.5-fold, A2BAR increased 3.7-fold and A3AR decreased 70% as compared with that of healthy gingiva.CONCLUSION: Inflammation of the gingival tissue is associated with (1) an unchanged expression of A1AR, (2) an increased expression of A2AAR and A2BAR, and (3) a decreased expression of A3AR. Logistic regression analysis indicated that the change in the expression patterns can be used to diagnose/predict periodontitis. This finding indicates that the adenosine receptor expression profile is changed in periodontitis with the potential for future clinical application.

AB - OBJECTIVE: To elucidate the aetiology of periodontitis, this study focused on the adenosine receptor (AR) expression profiles (A1AR, A2AAR, A2BAR and A3AR) in periodontal diseased tissues.METHODS: Adenosine receptor gene expression levels in human gingiva from 15 patients with healthy gingival tissues (control group) and 15 patients who exhibited severe chronic periodontitis (test group) were measured using quantitative reverse transcription-polymerase chain reaction (RT-PCR).RESULTS: The mRNA expression pattern changed in human chronic periodontitis: the A1AR decreased 20%, A2AAR increased 2.5-fold, A2BAR increased 3.7-fold and A3AR decreased 70% as compared with that of healthy gingiva.CONCLUSION: Inflammation of the gingival tissue is associated with (1) an unchanged expression of A1AR, (2) an increased expression of A2AAR and A2BAR, and (3) a decreased expression of A3AR. Logistic regression analysis indicated that the change in the expression patterns can be used to diagnose/predict periodontitis. This finding indicates that the adenosine receptor expression profile is changed in periodontitis with the potential for future clinical application.

KW - Chronic Periodontitis/genetics

KW - Alveolar Bone Loss/metabolism

KW - Humans

KW - Middle Aged

KW - Periodontal Attachment Loss/metabolism

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KW - Receptor, Adenosine A2A/analysis

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Gingivitis/metabolism

KW - Receptor, Adenosine A1/analysis

KW - Young Adult

KW - Receptors, Purinergic P1/analysis

KW - Aged, 80 and over

KW - Adult

KW - Female

KW - RNA, Messenger/analysis

KW - Aged

KW - Receptor, Adenosine A2B/analysis

KW - Gingival Hemorrhage/metabolism

KW - Real-Time Polymerase Chain Reaction

KW - Receptor, Adenosine A3/analysis

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M3 - Article

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SN - 1462-6446

VL - 14

SP - 113

EP - 120

JO - The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA)

JF - The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA)

IS - 2

ER -

Changes in mRNA expression of adenosine receptors in human chronic periodontitis.

Abstract

ASJC Scopus Subject Areas

Keywords

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