TY - CHAP
T1 - Capsaicin Pre-treatment Provides Neurovascular Protection Against Neonatal Hypoxic-Ischemic Brain Injury in Rats
AU - Khatibi, Nikan H.
AU - Jadhav, Vikram
AU - Charles, Shelton
AU - Chiu, Jeffrey
AU - Buchholz, John
AU - Tang, Jiping
AU - Zhang, John H.
N1 - Funding Information:
This study is partially supported by NIH NS043338 to J.H. Zhang, and NS060936 to J. Tang. Conflict of interest statement We declare that we have no conflict of interest.
PY - 2011/1/1
Y1 - 2011/1/1
N2 - Capsaicin, a transient receptor potential vanilloid 1 (TRPV1) agonist, has recently been shown to provide neuroprotection against brain injury in experimental adult models of cerebral ischemia. Accordingly, in this study, we investigated the way in which capsaicin-mediated TRPV1 modulation could attenuate damage in an experimental hypoxic-ischemic (HI) neonatal brain injury model. The Rice-Vannucci method was used in 10-day-old rat pups by performing unilateral carotid artery ligation followed by 2 h of hypoxia (8% O2 at 37°C). Capsaicin was administered intraperitoneally (0.2 mg/kg or 2.0 mg/kg) at 3 h pre-HI or 1 h post-HI. Post assessment included measurement of infarction volume at 24 and 72 h in addition to an assessment of the vascular dynamics of the middle cerebral artery (MCA) at 6 h post-HI. The results indicated that pre-treatment with capsaicin reduced infarction volume significantly with either low-dose or high-dose treatment. Pre-treatment also improved myogenic tone and decreased apoptotic changes in the distal MCA. We concluded that capsaicin pre-treatment may provide neurovascular protection against neonatal HI. © 2011 Springer-Verlag/Wien.
AB - Capsaicin, a transient receptor potential vanilloid 1 (TRPV1) agonist, has recently been shown to provide neuroprotection against brain injury in experimental adult models of cerebral ischemia. Accordingly, in this study, we investigated the way in which capsaicin-mediated TRPV1 modulation could attenuate damage in an experimental hypoxic-ischemic (HI) neonatal brain injury model. The Rice-Vannucci method was used in 10-day-old rat pups by performing unilateral carotid artery ligation followed by 2 h of hypoxia (8% O2 at 37°C). Capsaicin was administered intraperitoneally (0.2 mg/kg or 2.0 mg/kg) at 3 h pre-HI or 1 h post-HI. Post assessment included measurement of infarction volume at 24 and 72 h in addition to an assessment of the vascular dynamics of the middle cerebral artery (MCA) at 6 h post-HI. The results indicated that pre-treatment with capsaicin reduced infarction volume significantly with either low-dose or high-dose treatment. Pre-treatment also improved myogenic tone and decreased apoptotic changes in the distal MCA. We concluded that capsaicin pre-treatment may provide neurovascular protection against neonatal HI. © 2011 Springer-Verlag/Wien.
KW - Capsaicin
KW - Neonatal hypoxia ischemia
KW - Transient receptor potential vanilloid 1 (TRPV1)
KW - Animals, Newborn
KW - Neuroprotective Agents/administration & dosage
KW - Phosphopyruvate Hydratase/metabolism
KW - Drug Administration Schedule
KW - Rats
KW - TRPV Cation Channels/metabolism
KW - Middle Cerebral Artery/drug effects
KW - Brain Infarction/etiology
KW - Tetrazolium Salts
KW - Animals
KW - Infarction, Middle Cerebral Artery/pathology
KW - Analysis of Variance
KW - Capsaicin/administration & dosage
KW - Time Factors
KW - von Willebrand Factor/metabolism
KW - Gene Expression Regulation/drug effects
KW - Disease Models, Animal
UR - http://europepmc.org/articles/PMC3569063
UR - https://www.mendeley.com/catalogue/40378a40-0dc0-34f1-9d61-efefa255e9e5/
U2 - 10.1007/978-3-7091-0693-8_38
DO - 10.1007/978-3-7091-0693-8_38
M3 - Chapter (peer-reviewed)
C2 - 21725760
SN - 9783709106921
SN - 978-3-7091-2007-1
T3 - Acta Neurochirurgica, Supplementum
SP - 225
EP - 230
BT - Intracerebral Hemorrhage Research
PB - Springer-Verlag Wien
ER -