Acute and delayed Deferoxamine treatment attenuates long-term Sequelae after germinal matrix hemorrhage in neonatal rats

Damon Klebe, Paul R. Krafft, Clotilde Hoffmann, Tim Lekic, Jerry J. Flores, William Rolland, John H. Zhang

Research output: Contribution to journalArticlepeer-review

Abstract

BACKGROUND AND PURPOSE - : This study investigated if acute and delayed deferoxamine treatment attenuates long-term sequelae after germinal matrix hemorrhage (GMH). METHODS - : Bacterial collagenase (0.3 U) was infused intraparenchymally into the right hemispheric ganglionic eminence in P7 rat pups to induce GMH. GMH animals received either deferoxamine or vehicle twice a day for 7 consecutive days. Deferoxamine administration was initiated at either 1 hour or 72 hours post-GMH. Long-term neurocognitive deficits and motor coordination were assessed using Morris water maze, rotarod, and foot fault tests between day 21 to 28 post-GMH. At 28 days post-GMH, brain morphology was assessed and extracellular matrix protein (fibronectin and vitronectin) expression was determined. RESULTS - : Acute and delayed deferoxamine treatment improved long-term motor and cognitive function at 21 to 28 days post-GMH. Attenuated neurofunction was paralleled with improved overall brain morphology at 28 days post-GMH, reducing white matter loss, basal ganglia loss, posthemorrhagic ventricular dilation, and cortical loss. GMH resulted in significantly increased expression of fibronectin and vitronectin, which was reversed by acute and delayed deferoxamine treatment. CONCLUSIONS - : Acute and delayed deferoxamine administration ameliorated long-term sequelae after GMH. © 2014 American Heart Association, Inc.
Original languageEnglish
Pages (from-to)2475-2479
Number of pages5
JournalStroke
Volume45
Issue number8
DOIs
StatePublished - Aug 2014

ASJC Scopus Subject Areas

  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine
  • Advanced and Specialized Nursing

Keywords

  • deferoxamine
  • extracellular matrix proteins
  • Animals, Newborn
  • Brain/drug effects
  • Intracranial Hemorrhages/drug therapy
  • Animals
  • Time Factors
  • Rats
  • Deferoxamine/administration & dosage
  • Maze Learning/drug effects
  • Motor Activity/drug effects
  • Disease Models, Animal

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