TY - JOUR
T1 - A simple method for screening porphyrin secretion by colonies of Escherichia coli
AU - Javor, George T.
AU - Kim, Henry
N1 - Summary. Escherichia coli secretes porphyrins when exposed to 20 mM 1-thioglycerol. A method was devised to isolate mutants, which do not excrete porphyrins in
PY - 1988/12/1
Y1 - 1988/12/1
N2 - Escherichia coli secretes porphyrins when exposed to 20 mM 1-thioglycerol. A method was devised to isolate mutants, which do not excrete porphyrins in the presence of thiols. DEAE-Sephadex beads were incorporated into plates growing colonies of E. coli. The secreted porphyrins on these plates formed a fluorescent halo around each colony, when the plates were viewed under long-wave ultraviolet light. Mutants were found, that formed either halo-less colonies or a colony with excess halo. This method of incorporating immobilized ion exchangers into plates may be useful in isolating non-secretor or super-secretor mutants of a variety of organisms.
AB - Escherichia coli secretes porphyrins when exposed to 20 mM 1-thioglycerol. A method was devised to isolate mutants, which do not excrete porphyrins in the presence of thiols. DEAE-Sephadex beads were incorporated into plates growing colonies of E. coli. The secreted porphyrins on these plates formed a fluorescent halo around each colony, when the plates were viewed under long-wave ultraviolet light. Mutants were found, that formed either halo-less colonies or a colony with excess halo. This method of incorporating immobilized ion exchangers into plates may be useful in isolating non-secretor or super-secretor mutants of a variety of organisms.
KW - Mutant selection
KW - Porphyrin metabolism
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U2 - 10.1111/j.1574-6968.1988.tb03176.x
DO - 10.1111/j.1574-6968.1988.tb03176.x
M3 - Article
SN - 0378-1097
VL - 56
SP - 195
EP - 197
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 2
ER -