A radioimmunoassay procedure for quantitating parotid hormone

Jean Marc Tieche; John Leonora; Lee S. Berk; Ralph R. Steinman

doi:10.1016/0006-291X(81)91882-9

A radioimmunoassay procedure for quantitating parotid hormone

Jean Marc Tieche
, John Leonora
, Lee S. Berk
, Ralph R. Steinman

Research output: Contribution to journal › Article › peer-review

Abstract

The methodology and characterization of a double antibody radioimmunoassay (RIA) for quantitating parotid hormone (PH) in biological fluids are reported. A specific antiserum against PH was raised in rabbits using PH conjugated to human serum albumin. Its binding capacity and association constant were 22 μg/ml and Ka = 1.01 × 10^-12 M^-1, respectively. The sensitivity of the RIA was 1.5 pg PH when a sequential incubation schedule was used. This RIA makes possible the quantitation of PH in biological fluids and tissue extracts.

Original language	English
Pages (from-to)	154-162
Number of pages	9
Journal	Biochemical and Biophysical Research Communications
Volume	98
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(81)91882-9
State	Published - Jan 15 1981

ASJC Scopus Subject Areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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abstract = "The methodology and characterization of a double antibody radioimmunoassay (RIA) for quantitating parotid hormone (PH) in biological fluids are reported. A specific antiserum against PH was raised in rabbits using PH conjugated to human serum albumin. Its binding capacity and association constant were 22 μg/ml and Ka = 1.01 × 10-12 M-1, respectively. The sensitivity of the RIA was 1.5 pg PH when a sequential incubation schedule was used. This RIA makes possible the quantitation of PH in biological fluids and tissue extracts.",

author = "Tieche, \{Jean Marc\} and John Leonora and Berk, \{Lee S.\} and Steinman, \{Ralph R.\}",

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AU - Tieche, Jean Marc

AU - Leonora, John

AU - Berk, Lee S.

AU - Steinman, Ralph R.

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N2 - The methodology and characterization of a double antibody radioimmunoassay (RIA) for quantitating parotid hormone (PH) in biological fluids are reported. A specific antiserum against PH was raised in rabbits using PH conjugated to human serum albumin. Its binding capacity and association constant were 22 μg/ml and Ka = 1.01 × 10-12 M-1, respectively. The sensitivity of the RIA was 1.5 pg PH when a sequential incubation schedule was used. This RIA makes possible the quantitation of PH in biological fluids and tissue extracts.

AB - The methodology and characterization of a double antibody radioimmunoassay (RIA) for quantitating parotid hormone (PH) in biological fluids are reported. A specific antiserum against PH was raised in rabbits using PH conjugated to human serum albumin. Its binding capacity and association constant were 22 μg/ml and Ka = 1.01 × 10-12 M-1, respectively. The sensitivity of the RIA was 1.5 pg PH when a sequential incubation schedule was used. This RIA makes possible the quantitation of PH in biological fluids and tissue extracts.

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JO - Biochemical and Biophysical Research Communications

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A radioimmunoassay procedure for quantitating parotid hormone

Abstract

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